基于mt-roGFP1荧光探针研究几种植物源除草化合物对拟南芥根尖细胞氧化还原电位的影响

The effect of several botanical herbicidal chemicals on redox potential of Arabidopsis thaliana root tip cells based on mt-roGFP1 fluorescence probe

[目的]本文旨在研究源于秃杉的秃杉素、台湾脂素H(Taiwanin H)和台湾脂素E(Taiwanin E)等新型除草化合物对拟南芥根尖细胞氧化还原电位的影响。[方法]采用mt-roGFP1标记的拟南芥转基因植株测定了不同浓度的新型除草化合物处理拟南芥后,对其根的根冠、分生区、过渡区和伸长区细胞氧化还原电位的影响。[结果]不同浓度的秃杉素、台湾脂素H和台湾脂素E均能降低根尖细胞氧化还原电位,且在一定范围内,随着化合物浓度的增加,对根分生区细胞氧化还原电位的影响越大。0.1、1、5和10μg·mL^(-1)的秃杉素分别处理mt-roGFP1标记的拟南芥转基因植株后,根分生区细胞平均氧化还原电位变化最大差值分别为13.06、18.59、27.74和29.92 mV;1、5和10μg·mL^(-1)台湾脂素H和台湾脂素E分别处理mt-roGFP1标记的拟南芥转基因植株后,其根分生区细胞平均氧化还原电位变化最大差值分别为12.21、15.72和22.67 mV以及14.71、24.03和28.69 mV。[结论]在一定浓度范围内,秃杉素、台湾脂素H和台湾脂素E的浓度越大,拟南芥根分生区细胞氧化还原电位变化差值越大,具有较好的剂量-效应关系。这对利用mt-roGFP1荧光探针技术研究除草化合物对根细胞线粒体的作用提供了基础,同时为建立除草活性化合物快速筛选平台提供理论依据。

[Objectives]The paper aimed to study the effects of new herbicidal compounds derived from TSC-3,Taiwanin H and Taiwanin E on the redox potential of Arabidopsis root tip cells.[Methods]The effects of different concentrations of new herbicides on the redox potential of root cap,meristematic zone,transition zone and elongation zone of Arabidopsis thaliana were determined by using mt-roGFP1 A.transgenic.[Results]Different concentrations of TSC-3,Taiwanin H and Taiwanin E could reduce the redox potential of root tip cells of the mt-roGFP1 labeled transgenic Arabidopsis thaliana plants,with the increase of the concentration of the compound,the greater the effect on the redox potential of the meristem zone of mt-roGFP1 labeled transgenic A.thaliana plants.After being treated with TSC-3 at 0.1,1,5 and 10μg·mL^(-1),the mean values of the maximum variation in redox potential changes in root cells of the meristem zone were 13.06,18.59,27.74 and 29.92 mV,respectively.When treated with Taiwanin H and Taiwanin E at 1,5 and 10μg·mL^(-1),the mean values of the maximum variation of redox potential in root cells of the meristem zone were 12.21,15.72,22.67 mV and 14.71,24.03,28.69 mV,respectively.[Conclusions]In a certain range,the greater the concentration of TSC-3,Taiwanin H and Taiwanin E,the greater of the redox potential changes in root cells of the meristem zone.It indicated that the redox potential response of A.thaliana root tip cells to these herbicidal compounds was dose-response related.Our results provide a basis for the study of the mechanism of novel plant-derived herbicides on root cell mitochondria by roGFP1 fluorescence probe technology,and they also provide theoretical basis for the establishment of a rapid screening platform for herbicidal compounds.