TET 2基因单核苷酸多态性与急性心肌梗死易感性和临床特征及预后的相关性分析

Correlation analysis between TET2 single nucleotide polymorphism and susceptibility,clinical characteristics and prognosis of acute myocardial infarction

目的探讨TET 2基因单核苷酸多态性(SNP)与急性心肌梗死(AMI)易感性、临床特征及预后的相关性,为临床探究AMI发病的分子遗传学提供理论基础及实验依据。方法选取2022年1-10月在承德医学院附属医院确诊的AMI患者及同期相匹配的健康体检人群各100例为研究对象,分为AMI组和对照组。统计TET 2基因2个SNP位点rs7670522、rs6839705基因型(AA、AG、GG)、等位基因(A、G)在AMI不同分类易感性(对照组、AMI组)、临床特征(有无临床典型症状、严重并发症)、预后(死亡、存活)中的分布规律。采用多因素Logistic回归方法分析AMI患者预后的影响因素。结果AMI组rs7670522、rs6839705位点AA基因型、A等位基因频率均高于对照组,差异均有统计学意义(均P<0.05)。AMI患者中有典型症状组rs7670522、rs6839705位点AA基因型、A等位基因频率均明显高于无典型症状组,有严重并发症组rs7670522、rs6839705位点AA基因型、A等位基因频率均明显高于无严重并发症组(均P<0.05)。AMI患者中死亡组rs7670522、rs6839705位点AA基因型、A等位基因频率均明显高于存活组(均P<0.05)。多因素Logistic回归分析结果显示,TET2 rs7670522、TET2 rs6839705均与AMI患者预后密切相关(比值比=3.244、3.095,P=0.004、0.006)。结论TET 2基因2个SNP位点rs7670522、rs6839705多态性与AMI易感性、临床特征及预后存在相关性,基因型AA、等位基因A均是导致患者预后不良的关键因素。

Objective To investigate the correlation between single nucleotide polymorphism(SNP)of TET2 and acute myocardial infarction(AMI)susceptibility,main clinical characteristics and prognosis,and to provide theoretical basis and experimental basis for clinical research on molecular genetics of AMI.Methods Totally 100 cases of AMI patients diagnosed in Affiliated Hospital of Chengde Medical University from January to October 2022 and 100 cases of matched healthy people in the same period were collected as research objects,divided to AMI group and control group.The distribution patterns of rs7670522 and rs6839705 genotypes(AA,AG,GG)and alleles(A,G)of two SNP loci of TET2 gene in AMI susceptibility(control group and AMI group),clinical characteristics(whether presented clinical severe symptoms and serious complications)and prognosis(death and survival)were analyzed.Multivariate logistic regression method was used to analyze the influencing factors of prognosis in AMI patients.Results Genotype AA and allele A of rs7670522 and RS6839705 in AMI group were higher than those in control group(all P<0.05).Genotype AA and allele A of rs7670522 and rs6839705 in the group with typical symptoms were significantly higher than those in the group without typical symptoms(all P<0.05).Genotype AA and allele A of rs7670522 and rs6839705 were significantly higher in the group with severe complications than those in the group without serious complications(all P<0.05).Genotype AA and allele A of rs7670522 and RS6839705 were significantly higher in the death group than those in the survival group(all P<0.05).Multivariate Logistic regression analysis showed that both TET2 rs7670522 and TET2 rs6839705 were closely related to the prognosis of AMI patients(odds ratio=3.244,3.095;P=0.004,0.006).Conclusions The polymorphism of rs7670522 and rs6839705 SNP loci of TET2 gene is correlated with the susceptibility to acute myocardial infarction,clinical features and prognosis.Genotype AA and allele A are the key factors leading to poor prognosis of patients.