亚硝态氮充足下乙酸钠对厌氧氨氧化/反硝化的影响

Effect of Sodium Acetate on ANAMMOX Coupled Denitrification System with Sufficient Nitrite Nitrogen

通过连续流实验,研究了在亚硝态氮充足的条件下乙酸钠有机物对厌氧氨氧化(ANAMMOX)耦合反硝化系统(SAD)的影响。结果表明,当乙酸钠浓度(以COD计)为20 mg/L时,耦合系统脱氮性能良好;而当乙酸钠浓度增至50 mg/L时,ANAMMOX受到严重抑制,系统脱氮性能受到严重影响。进一步通过高通量生物测序研究颗粒污泥的微生物菌群变化。随着乙酸钠浓度的增加,颗粒污泥的主要厌氧氨氧化菌属Candidatus Kuenenia所占比例由20.42%降为2.30%,厌氧氨氧化菌的生物密度逐渐降低,反硝化菌的生物密度逐渐升高,厌氧氨氧化菌处于劣势。

This paper investigated the effect of sodium acetate on continuous-flow anaerobic ammonia oxidation(ANAMMOX)coupled denitrification system(SAD)under the condition of sufficient nitrite nitrogen.When the concentration of sodium acetate(COD)was 20 mg/L,satisfactory nitrogen removal performance of the coupled system was obtained.When the concentration of sodium acetate was increased to 50 mg/L,ANAMMOX was severely inhibited,and the nitrogen removal performance of the system was significantly affected.The change in microbial community of the granular sludge was further analyzed by high-throughput sequencing.With the increase of sodium acetate concentration,the relative abundance of Candidatus Kuenenia which was the main ANAMMOX bacteria(AAOB)in granular sludge,decreased from 20.42%to 2.30%,the biological density of AAOB gradually decreased,and the biological density of denitrifying bacteria gradually increased,indicating that the ANAMMOX bacteria was disadvantageous in the system.