臭氧催化氧化/BAF深度处理高含硫气田采出水

Catalytic Ozonation/Biological Aerated Filter Combined Process for Advanced Treatment of Effluent from High Sour Gas Field

为了探索高盐采出水深度处理机理,基于生产规模装置,监测关键水质指标,探寻水质变化规律;开展高通量测序和荧光定量PCR分析,探索曝气生物滤池(BAF)中细菌、氨氧化细菌(AOB)和氨氧化古菌(AOA)特征。结果表明,在进水氯离子、COD和氨氮浓度分别为9912、223和44.0 mg/L条件下,当臭氧投加量为200~350 mg/L、BAF曝气量为150~300 m^(3)/h时,臭氧催化氧化和BAF对COD的去除率分别为49.3%和15.8%,对氨氮的去除率分别为38.2%和54.3%;BAF出水COD和氨氮的浓度分别为77.8和3.29 mg/L,满足下游工艺要求。氨氮通过硝化作用全部转化为亚硝态氮或硝态氮,没有反硝化代谢。系统中的细菌主要为红杆菌、鞘脂菌、固氮弓菌、红环菌和Planktosalinus;AOB的绝对优势菌是亚硝化单胞菌;AOA的优势菌是Nitrososphaera和Nitrosocosmicus,均隶属于奇古菌门。细菌、AOB和AOA的含量分别为(0.63~1.33)×10^(9)、(2.00~4.21)×10^(8)和(4.14~9.84)×10^(4)拷贝数/g填料;细菌和AOB含量丰富,AOA含量较低,说明细菌和AOB在有机物和氨氮降解过程中发挥主力作用。

This paper explored the variation in water quality by monitoring the key water quality indexes,investigated the characteristics of bacteria such as AOB and archaea such as AOA in biological aerated filter(BAF)by high-throughput sequencing and fluorescence quantitative PCR,so as to reveal the mechanism of a full-scale catalytic ozonation/BAF process for advanced treatment of hypersaline effluent from a gas filed.When the chloride ion concentration,COD and ammonia nitrogen in influent were 9912 mg/L,223 mg/L and 44.0 mg/L,respectively,the ozone dosage was in the range of 200-350 mg/L and the BAF aeration rate was between 150 m^(3)/h and 300 m^(3)/h,the COD removal rates of ozone catalytic oxidation and BAF were 49.3%and 15.8%,respectively,and the removal rates of ammonia nitrogen were 38.2%and 54.3%,respectively.The COD and ammonia nitrogen in effluent from BAF were 77.8 mg/L and 3.29 mg/L,respectively,which met the influent requirements of downstream process.All ammonia nitrogen was converted to nitrite nitrogen or nitrate nitrogen through nitrification,and there was no denitrification.The dominant bacteria were Rhodobacter,Sphingobium,Azoarcus,Rhodocyclus and Planktosalinus.The most dominant AOB genus was Nitrosomonas.The dominant AOA genera were Nitrososphaera and Nitrosocosmicus,both of which belong to phylum of Thaumarchaeota.The contents of bacteria,AOB and AOA were(0.63-1.33)×10^(9)copies/g filler,(2.00-4.21)×10^(8)copies/g filler and(4.14-9.84)×10^(4)copies/g filler,respectively.The content of bacteria and AOB was abundant,while the content of AOA was low,indicating that bacteria and AOB played a major role in the degradation of organic contaminants and ammonia nitrogen.