摘要
本实验合成并纯化得到两亲性聚羟基脂肪酸酯颗粒表面结合蛋白(PhaP),将其修饰在前期制备的姜黄素纳米胶囊(Cur@PHA)表面,通过表面改性有效提高了纳米胶囊的分散性能;然后对表面改性姜黄素纳米胶囊(Cur-PhaP@PHA)的形貌、稳定性、药物释放性能及体外细胞毒性进行了表征和分析。结果表明:Cur-PhaP@PHA表面形态和球形度好,光稳定性能优,具有良好的药物缓释、控释作用,表现出更优的药物吸收和生物利用度。该综合实验设计由产业需求出发,帮助学生从产业应用、技术创新的角度分析解决问题,有利于提高学生综合创新能力。
In this experiment,amphiphilice polyhydroxyalkanoate(PHA)particle surface binding protein(PhaP)was synthesized and purified.PhaP was effectively modified on the surface of curcumin nanosphere(Cur@PHA),and the dispersion performance of nanosphere was effectively improved by surface modification.The morphology,stability,drug release and in vitro cytotoxicity of the drug delivery system of Cur-PhaP@PHA were characterized and analyzed.The results showed that Cur-PhaP@PHA had good sphericity,excellent light stability,and sustained and controlled release.It also exhibited better drug absorption and bioavailability than free curcumin.This comprehensive experimental design is based on industrial demand,inspiring students to analyze and solve problems from the perspective of industrial application and technological innovation,which is conducive to improving students’comprehensive innovation ability.
作者
刘焕迪
杜洁
张红蕾
贾新超
张路路
李玮
LIU Huandi;DU Jie;ZHANG Honglei;JIA Xinchao;ZHANG Lulu;LI Wei(College of Chemistry and Materials Science,Hebei University,Baoding 071002,China;Chenguang Biotech Group Limited Co.,Ltd.,Handan 056000,China;Muqun Biotech Co.,Ltd.,Baoding 071002,China)
出处
《实验科学与技术》
2023年第4期118-123,共6页
Experiment Science and Technology
基金
国家自然科学基金(3210120332)
河北省高等教育教学改革研究与实践项目(2021GJJG001)
河北大学产学合作项目(横2020050)
河北大学产学合作项目(横2020160)。
