不同质量浓度MS-222对红尾副鳅麻醉效果的影响

Anesthetic effect of MS-222 at different mass concentrations on Paracobitis variegatus

【目的】探究不同质量浓度间氨基苯甲酸乙酯甲磺酸盐(MS-222)对红尾副鳅(Paracobitis variegatus)的麻醉效果及其生理行为特征,为鱼类野外活体样本采集、驯养和繁育等科研生产活动提供麻醉技术并丰富鱼类麻醉理论。【方法】采用静水生物测试法,设置35.0、45.0、60.0、70.0、85.0、110.0、140.0、150.0、180.0和210.0 mg/L共10组MS-222质量浓度进行红尾副鳅麻醉试验,检测不同质量浓度MS-222药浴红尾副鳅后的麻醉和复苏阶段经过时间及呼吸频率,筛选适宜红尾副鳅驯养、繁育和疾病防治的MS-222麻醉剂质量浓度。【结果】依据MS-222麻醉红尾副鳅后其不同呼吸频率和行为特征,可将红尾副鳅麻醉程度分为应激期、轻度镇静期、深度镇静期、轻度麻醉期、麻醉期和深度麻醉期(A1~A6期)6个麻醉阶段,将复苏过程分为复苏期1~复苏期4(R1~R4期)4个复苏等级;10个质量浓度MS-222麻醉均能使红尾副鳅达到A6阶段,且均能复苏至R4等级。相关分析结果表明,MS-222质量浓度与麻醉时间呈显著负相关(R^(2)=-0.6003)(P<0.05,下同),与复苏时间呈显著正相关(R^(2)=0.7440);麻醉剂量均显著影响红尾副鳅的麻醉与复苏。各质量浓度MS-222处理红尾副鳅的呼吸频率均呈波浪式变化,麻醉和复苏阶段的呼吸频率均存在差异。在充氧条件下,35.0~210.0 mg/L MS-222处理红尾副鳅的复苏率均达100.0%。【结论】不同质量浓度MS-222处理红尾副鳅在麻醉和复苏阶段的呼吸频率均存在差异,理想的麻醉浓度区间为45.0~180.0 mg/L,110.0~180.0 mg/L的MS-222可用于红尾副鳅短时麻醉,45.0~85.0 mg/L的MS-222可用于红尾副鳅较长时间麻醉。

【Objective】This paper investigated the physiological behavior characteristics of Paracobitis variegatus and the anesthetic effects of various concentrations of 3-aminobenzoic acid ethyl ester methanesulfonate(MS-222)in order to provide anesthesia technical support for the collection of live fish samples in the wild,fish domestication and breeding,and to advance the theory of fish anesthesia.【Method】Ten groups of MS-222 were set up with the mass concentrations of 35.0,45.0,60.0,70.0,85.0,110.0,140.0,150.0,180.0 and 210.0 mg/L to carry out the anaesthesia test of P.variegatus,using static water bioassay.Duration of anesthesia and resuscitation,and the respiratory frequency of P.variegatus under MS-222 immersion baths of different mass concentrations was measured so as to screen mass concentrations of MS-222 anesthetics suitable for domestication,breeding and disease control of P.variegatus.【Result】Based on different respiratory frequencies and behavioral characteristics after treatment of MS-222 of P.variegatus,the anesthesia degrees could be divided into 6(A1-A6)anesthesia stages:irritability,slight sedation,deep sedation,slight anesthesia,anesthesia and deep anesthesia,and resuscitation duration was divided into 4 resuscitation levels,namely resuscitation stage 1-resuscitation stage 4(R1-R4).All the 10 mass concentrations of MS-222 could make P.variegatus reach the A6(deep anesthesia),and resuscitate to the R4 level.According to correlation analysis,significant negative correlations were found between the mass concentration of MS-222 and anesthesia time(R^(2)=0.6003)(P<0.05,the same below),and the mass concentration of MS-222 significantly positively correlated with resuscitation time(R^(2)=0.7440);the anesthetic dose significantly affected the anesthesia and resuscitation of P.variegatus.Different mass concentrations of MS-222 had a wavy variation in the respiratory frequency of treated P.variegatus.There were differences in respiratory frequency between anesthesia stage and resuscitation stage with different mass concentrations of MS-222.Under oxygenation,the resuscitation rate of P.variegatus treated with MS-222 at the concentration of 35.0-210.0 mg/L reached 100.0%.【Conclusion】The respiratory frequency of P.variegatus treated with different mass concentrations of MS-222 is discrepant during the anesthesia and the resuscitation stages.The ideal anesthetic concentration of MS-222 for P.variegatus is 45.0-180.0 mg/L.MS-222 at the concentration of 110.0-180.0 mg/L can be used for short-term anesthesia of P.variegatus,while MS-222 at the concentration of 45.0-85.0 mg/L can be used for long-term anesthesia of P.variegatus.