银杏叶和柿子叶黄酮类化合物提取条件优化研究

Optimization of Extraction Conditions of Flavonoids from Ginkgo BilobaLeaves and Diospyros Kaki Leaves

以黄酮类化合物提取率为评价指标,考察料液比、超声波功率及水浴时间对银杏叶和柿子叶提取的影响.采用超声波辅助水溶液提取法对银杏叶及柿子叶进行提取,通过单因素试验确定料液比、超声波功率及水浴时间的较佳范围;在此基础上设计3因素3水平正交试验优化试验条件.通过正交试验结果确定,银杏叶的最佳提取工艺为料液比1:20,超声波功率144 W,水浴时间1.0 h,在此条件下,黄酮类化合物的提取率为4.18%;柿子叶的最佳提取工艺为料液比1:35,超声波功率126 W,水浴时间2.0 h,在此条件下,黄酮类化合物的提取率为2.54%.通过该工艺银杏叶和柿子叶黄酮类化合物提取率较高,为银杏叶和柿子叶提取物应用于化妆品行业提供参考.

Taking the extraction yield of flavonoids as the evaluation index,the effects of the ratio of solid to liquid,ultrasonic power and water bath time on the extraction of Ginkgo biloba and Diospyros kaki leaves were investigated.The extraction of Ginkgo biloba and Diospyros kaki leaves was carried out by ultrasonic assisted aqueous extraction method.And the better range of material-liquid ratio,ultrasonic power and water bath time was determined by single factor test.On this basis,the orthogonal experiment with 3-factors and 3-levels is designed to optimize the experimental conditions.The optimal extraction process of Ginkgo biloba was determined by orthogonal test results as follows:solid-liquid ratio 1∶20,ultrasonic power 144 W,and water bath time 1.0 h.Under this condition,the extraction rate offlavonoids was 4.18%.The optimal extraction technology of Diospyros kaki leaves was the solid-liquid ratio of 1∶35,the ultrasonic power of 126 W,and the water bath time of 2.0 h.Under this condition,the extraction rate offlavonoids was 2.54%.It lays a foundation for the application of Ginkgo biloba and Diospyros kaki leaf extract in cosmetics industry.