全血和血清降钙素原检测方法的临床研究

Clinical study on the detection method of procalcitonin in whole blood and serum

目的 探讨采用酶联免疫荧光法检测血清降钙素原(PCT)与采用免疫层析法检查全血PCT的临床价值。方法 采集2022年1-3月该院住院患者的乙二胺四乙酸二钾抗凝全血(96份)及促凝血标本(96份)。采用酶联免疫荧光法和免疫层析法分别检测血清和全血PCT水平,并分析其检测结果的相关性和一致性。结果 血清PCT水平为(5.88±0.89)ng/mL,全血PCT水平为(5.56±0.84)ng/mL,差异无统计学意义(t=0.25,P>0.05)。血清和全血PCT检测结果具有良好的线性关系(r=0.974,P<0.05)和一致性。结论 采用免疫层析法检测全血PCT与采用酶联免疫荧光法检测血清PCT具有较好的一致性。

Objective To explore the clinical value of enzyme-linked immunofluorescence for detecting serum procalcitonin(PCT)and immunochromatography for detecting whole blood PCT.Methods The EDTA-K 2 anticoagulant whole blood(96 samples)and procoagulant samples(96 samples)from hospitalized patients in the hospital from January to March 2022 were collected.Serum and whole blood PCT levels were detected by enzyme-linked immunofluorescence and immunochromatography,and the correlation and consistency of their detection results were analyzed.Results The serum PCT level was(5.88±0.89)ng/mL,and the whole blood PCT level was(5.56±0.84)ng/mL,with no statistically significant difference(t=0.25,P>0.05).The results of serum and whole blood PCT detection showed a good linear relationship(r=0.974,P<0.05)and consistency.Conclusion There is good consistency between using immunochromatography to detect whole blood PCT and using enzyme-linked immunofluorescence to detect serum PCT.