137份云南省地方稻抗白叶枯病多菌系多基因鉴定

Multi-bacterial lines and multi-gene identification of the bacterial blight pathogen in 137 rice landraces from Yunnan Province,China

为选育水稻抗性品种和发掘抗白叶枯病基因,以来自中国、日本和菲律宾的9株革兰氏阴性菌黄单胞菌水稻变种Xanthomonas oryzae pv.oryzae菌株为供试菌株评价137份云南省地方稻种质资源对白叶枯病菌的抗性,同时检测供试材料中含14个已克隆抗白叶枯病基因的情况。结果显示,供试材料中至少对1株黄单胞菌水稻变种菌株有抗性的材料有104份,抗1、2、3、4、5、6、7、8和9株黄单胞菌水稻变种菌株的材料分别有25、19、14、19、11、6、5、2和3份;其中白杂一号、珍白18、麻旱谷、老来红、晋糯、香谷、毫冷薅、齐头蚂蚱谷、九谷和来之不易10份材料抗谱较广。大部分抗性材料携带1~7个白叶枯病抗性基因或其同源基因,未检测到含有Xa7、xa13、Xa21和Xa45(t)基因或其同源基因的材料。供试材料携带的抗性基因数量与抗性水平均呈极显著相关。

To breed resistance rice varieties and mining for the bacterial blight resistance genes,nine represent Xanthomonas oryzae pv.oryzae(Xoo)strains from China,Japan and Philippines were inoculated with 137 rice landraces from Yunnan Province to evaluate the phenotypic resistance to bacterial blight,and 14 cloned bacterial blight resistance genes contained in the tested materials were tested.The results showed that 104 materials were resistant to at least one Xoo strain,and the numbers of materials resistant to one through nine Xoo strains were 25,19,14,19,11,6,5,2 and 3 accessions,respectively.Among them,materials Baizayihao,Zhenbai 18,Mahangu,Laolaihong,Jinnuo,Xianggu,Haolenghao,Qitoumazhagu,Jiugu,and Laizhibuyi 10 had a broad resistance spectrum.Most of the resistance materials carried one to seven bacterial blight resistance genes or their homologs examined,and four resistance allelic fragments of Xa7,xa13,Xa21 and Xa45(t)were not detected in the resistant materials.The number of resistance genes carried by the tested materials was all significantly associated with the resistance level.