摘要
介绍一个由科研成果转化的分析化学综合设计性实验“基于指示剂置换法的肝素含量测定”。本实验基于指示剂置换法的分析原理,以甲基紫精(MV^(2+))作为肝素受体,以8-羟基芘-1,3,6-三磺酸三钠盐(pyranine)作为光学指示剂,基于MV^(2+)和肝素、pyranine之间结合能力的不同,利用加入肝素后pyranine-MV^(2+)体系的紫外-可见光谱吸光度的变化,实现对肝素的定量分析。
Herein we describe a comprehensive experiment designed for laboratory teaching of analytical chemistry.The experiment was developed based on the results of one of our recent studies.Methyl viologen(MV^(2+))and 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt(pyranine)were used as the heparin receptor and optical indicator,respectively.Once heparin was introduced in the reaction system,competitive binding led to pyranine displacement,thereby resulting in changes in the UV-Vis absorbance of the pyranine/MV^(2+)system.Using the changes in UV-Vis absorption as the output signal,heparin concentration can be accurately measured using the IDA-based method.
作者
张自品
赵祖志
Zipin Zhang;Zuzhi Zhao(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;School of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China)
出处
《大学化学》
CAS
2023年第8期240-247,共8页
University Chemistry
基金
安徽省自然科学基金面上项目(2008085MH293)
安徽省高校自然科研项目重点项目(2022AH050459)
安徽省2020年省级质量工程项目(2020MOOC265)
安徽医科大学国际教育类教改项目(gjjyxm202011)。
关键词
分析化学
综合设计性实验
指示剂置换法
肝素
Analytical chemistry
Comprehensive design experiment
Indicator displacement assay(IDA)
Heparin