电针上巨虚联合穴位注射西咪替丁对吲哚美辛致胃溃疡大鼠抗氧化酶活力、胃泌素、表皮生长因子及前列腺素E_(2)含量的影响

Effects of electroacupuncture at Shangjuxu combined with acupoint injection of Cimetidine on antioxidant enzyme activity,gastrin,epidermal growth factor and prostaglandin E_(2) contents in gastric ulcer rats induced by Indomethacin

目的:评价电针上巨虚联合穴位注射西咪替丁对非甾体抗炎药(NSAIDs)致大鼠胃溃疡的增效作用及针药协同机理。方法:32只SD大鼠,随机分为空白组、模型组、上巨虚组、非经非穴组,每组8只。以吲哚美辛(35 mg/kg,腹腔注射)诱导大鼠急性胃溃疡,上巨虚组、非经非穴(肌肉点)组采用电针刺激6 d后造模,并在造模后1 h分别于上巨虚和肌肉点注射西咪替丁(50 mg/kg)进行干预。观察造模后7 h各组大鼠胃溃疡程度,检测胃组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及血清表皮生长因子(EGF)、胃泌素(GAS)、前列腺素(PGE_(2))含量差异。结果:与模型组比较,干预两组的大鼠胃溃疡指数、血清GAS、PGE_(2)含量明显降低,EGF含量明显升高(P<0.05,P<0.01);上巨虚组大鼠胃组织病理评分明显降低(P<0.01),胃组织GSH-Px活力明显提高(P<0.05)。与非经非穴组比较,上巨虚组大鼠胃溃疡指数、血清GAS、PGE_(2)含量降低,血清EGF含量升高,差异有统计学意义(P<0.05,P<0.01);胃组织病理评分降低,胃组织GSH-Px活力有升高趋势,但差异无统计学意义。结论:对吲哚美辛所致大鼠胃溃疡,电针上巨虚联合穴位注射西咪替丁具有增效和协同作用。

Objective:To evaluate the synergistic effect of electroacupuncture at Shangjuxu combined with acupoint injection of Cimetidine on gastric ulcer induced by intraperitoneal injection of nonsteroidal anti-inflammatory drugs(NSAIDs)in rats and the synergistic mechanism of acupuncture and medicine.Methods:32 SD rats were randomly divided into blank group,model group,Shangjuxu group and non-meridian-non-acupoint group,with 8 rats in each group.Acute gastric ulcer was induced by Indomethacin(35 mg/kg through intraperitoneal injection)in rats.The Shangjuxu group and the non-meridian-non-acupoint(muscle points)group were stimulated by electroacupuncture for 6 days to establish the model,and Cimetidine(50 mg/kg)was injected into the Shangjuxu acupoint and muscle points respectively to intervene at 1 h after modeling.The degree of gastric ulcer in each group was observed 7 h after the model was made.The activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in gastric tissue and the contents of epidermal growth factor(EGF),gastrin(GAS)and prostaglandin(PGE2)in serum were detected.Results:Compared with the model group,the gastric ulcer index,serum GAS and PGE2 contents were significantly decreased and EGF contents were significantly increased in the two intervention groups(P<0.05,P<0.01).The gastric histopathological score of Shangjuxu group was significantly decreased(P<0.01),and the activity of GSH-Px in gastric tissue was significantly increased(P<0.05).Compared with the non-meridian-non-acupoint group,the gastric ulcer index,serum GAS and PGE2 contents of rats in Shangjuxu group were decreased,and serum EGF contents were increased,with the statistically significant differences(P<0.05,P<0.01).Gastric histopathological score decreased and gastric GSH-Px activity increased,but the difference was not statistically significant.Conclusion:Electroacupuncture at Shangjuxu combined with acupoint injection of Cimetidine has synergistic effect on gastric ulcer in rats induced by Indomethacin.