摘要
目的 了解淮安市新报告人感染HIV-1毒株的流行特征、亚型及分子传播网络特征。方法 采集淮安籍HIV-1确证阳性感染者血液标本,利用巢式(RT-)PCR方法扩增pol基因片段,测序并构建分子传播网络。结果 淮安市新报告人感染的HIV-1基因亚型有9种,分别为CRF01_AE (38.5%,47/122)、CRF07_BC (38.5%,47/122)、CRF67_01B (8.2%,10/122)、CRF68_01B (4.9%,6/122)、B (3.3%,4/122)、CRF08_BC (3.3%,4/122)、CRF55_01B (1.6%,2/122)、CRF85_BC (0.8%,1/122)和CRF52_01B (0.8%,1/122)。60条pol基因序列形成10个分子传播簇,入网率为49.2%,其中最大的分子传播网络由CRF07_BC基因型构成。logistic回归分析显示已婚有配偶(与丧偶相比,OR=0.242,95%CI:0.006-0.972)、同性传播(与异性传播相比,OR=2.113,95%CI:1.020-4.377)、基因型CRF07_BC (与其他重组型相比,OR=5.972,95%CI:1.655-21.554)更容易进入HIV-1分子传播网络并传播流行。结论 淮安市新报告人感染HIV-1的基因型具有多样性,分子传播网络中存在耐药株传播风险,因此应加强HIV-1的分子流行病学调查,全面开展基线耐药监测,防止耐药毒株在人群中的进一步传播和扩散。
Objective To gain more insights into epidemiologic characteristics,genotype and molecular transmissionnetwork characteristics of newly reported HIV-1 infected cases in Huai'an.Methods The samples of local confirmed cases in Huai'an were collected with epidemiological information.HIV-1 pol genes were amplified by nested(RT-)PCR from(RNA)DNA,followed by sequencing,aligning and molecular transmission-network construction.Results The study revealed the distributions of HIV-1 genotypes among newly reported HIV-1 infected cases in Huai'an were as folloWS:CRF01_AE(38.5%,47/122)、CRF07_BC(38.5%,47/122)、CRF67_01B(8.2%,10/122)、CRF68_01B(4.9%,6/122)、B(3.3%,4/122)、CRF08_BC(3.3%,4/122)、CRF55_01B(1.6%,2/122)、CRF85_BC(0.8%,1/122)andCRF52_01B(0.8%,1/122).A total of 60 pol gene sequences were used to generate 10 molecular transmission networks,with clustering rate 49.2%,and the largest transmission cluster was CRF07_BC.The logistic regression analysis results showed that married with spouse(compared with widowed spouse,OR=0.242,95%CI:0.0060-0.972),homosexual transmission(compared with heterosexual transmission,OR=2.113,95%CI:1.020-4.377),genotype CRF07_BC(compared with other CRFs,OR=5.972,95%CI:1.655-21.554)were more likely to enter the HIV-1 molecular transmission network and spread quickly in the network.Conclusion The diversity of HIV-1 among newly reported cases were circulating in Huai'an,and there was a risk of drug-resistant strains in molecular transmission networks.Frequency of HIV-1 molecular epidemiological surveillance and baseline drug resistance monitoring should be carried out to prevent the further spread of drug resistant strains in the population.
作者
燕清丽
周莹
杨鹏飞
方娟
张芹
庞艺秀
董正远
王美琦
YAN Qingli;ZHOU Ying;YANG Pengfei;FANG Juan;ZHANG Qin;PANG Yixiu;DONG Zhengyuan;WANG Meiqi(Huai'an Center for Disease Control and Prevention,Huai'an,Jiangsu,223001,China;Huaian Key Laboratory of Emergency Detection for Public Health Incidents;Department of AIDS/STD Prevention and Control,Jiangsu Center for Disease Prevention and Control;The Center for Disease Prevention and Control of the Seventh Division of Xinjiang Production and Construction Corps)
出处
《中国病原生物学杂志》
CSCD
北大核心
2023年第11期1264-1268,共5页
Journal of Pathogen Biology
基金
淮安市突发公共卫生事件应急检测重点实验室项目(No.HAP201906)。
关键词
新报告感染者
基因亚型
进化树
HIV-1分子传播网络
Newly reported HIV-1 infected cases
genotype
phylogenetic tree
HIV-1 molecular transmission network