Box-Behnken设计-响应面法优化桔梗醇提工艺及醇提物美白活性分析

Optimization of Alcohol Extraction Process of Platycodon grandiflorum by Box-Behnken Design-Response Surface Method and Analysis of Whitening Activity of Alcohol Extract

利用响应面法优化桔梗醇提工艺,并考察其美白活性。以提取时间、提取次数、溶媒量为影响因素,采用单因素考察和Box-Behnken响应面设计试验,优化桔梗醇提工艺;以酶抑制活性和抗氧化为指标考察提取物的美白活性。结果表明,桔梗的最佳乙醇提取工艺为提取次数3次,提取时间1 h,溶媒量8倍,该条件下制备的桔梗醇提物中桔梗皂苷D、熊果苷、桔梗总皂苷的提取率分别为0.122%±0.003%、0.128%±0.005%、0.582%±0.007%,且具有良好的酪氨酸酶抑制活性(92.39%)、透明质酸酶抑制活性(88.26%)、细胞黑色素抑制效果及自由基DPPH(90.19%)和ABTS^(+)(80.57%)清除活性。优化的桔梗醇提工艺稳定可靠,可操作性好,该工艺提取的桔梗醇提物具有良好的美白和抗氧化活性,为进一步研究桔梗生物活性及美白机制提供科学依据。

To optimize the alcohol extraction process of Platycodon grandiflorum by response surface methodology,then investigate its whitening activity.With extracting time,extracting times and solvent volumn as factors,the ethanol extraction process of Platycodon grandiflorum was optimized by single factor investigation and Box-Behnken response surface design.The optimal ethanol extracting technology was 8 times of solvent,extraction for 3 times,1 h each time.The extraction rates of platycodon D,arbutin,and total saponins in the ethanol extract were 0.122%±0.003%,0.128%±0.005%,and 0.582%±0.007%,respectively,and the extract had good tyrosinase inhibition activity(92.39%),hyaluronidase inhibition activity(88.26%),cell melanin inhibition effect,and DPPH·(90.19%),ABTS^(+)(80.57%)free radical scavenging activity.The optimal ethanol extraction process of Platycodon grandiflorum was stable,reliable and operable.The ethanol extract had good whitening and antioxidant activity,which would provide scientific basis for further research on biological activity and whitening mechanism of Platycodon grandiflorum.