基于层次分析-熵权法优化刺五加多组分超声提取工艺

Optimization of the Ultrasonic Extraction Process of Acanthopanax senticosus Multiple Components Based on a Coupling Methodology of Analytic Hierarchy Process and Entropy Weight Method

基于超高效液相色谱-质谱(ultra-high performance liquid chromatography-mass spectrometry,UPLC-MS/MS)定量方法以及多指标综合评价方法,对刺五加多组分超声提取工艺进行优化。开发UPLC-MS/MS定量方法,同时测定刺五加多组分(绿原酸、紫丁香苷、刺五加苷E、异嗪皮啶、咖啡酸、芝麻素)含量;再由层次分析法(analytic hierarchy process,AHP)及熵权法(entropy weight method,EWM)组建多指标综合评价方法—层次分析-熵权法(AHP-EWM);最终由单因素结合基于Box-Behnken设计(Box-Behnken design,BBD)的响应面法(response surface methodology,RSM)优化刺五加多组分超声提取工艺。结果表明,RSM优化所得刺五加多组分超声提取最佳工艺:超声功率780 W,超声时间17.5 min,乙醇体积分数57%,料液比1:40 g:mL,原料粒径80目。由此,刺五加根茎中绿原酸、紫丁香苷、刺五加苷E、异嗪皮啶、芝麻素的含量分别为2235.841±12.17、517.959±6.09、861.247±5.30、66.657±1.22、45.745±0.77、99.355±0.69μg/g,且基于AHP-EWM所得的综合得分为95.45±0.39,与预测理论值接近。刺五加定量分析方法与高效提取工艺的有效开发,为其资源利用以及药效基础研究奠定基础。

The multi-component ultrasonic extraction process of Acanthopanax senticosus was optimized via ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS/MS)quantitative method and the multi-index comprehensive evaluation method.An UPLC-MS/MS method for the simultaneous quantitative determination of multiple components(chlorogenic acid,syringin,eleutheroside E,isofraxidin,caffeic acid and sesamin)in rhizome of Acanthopanax senticosus was developed.Then,the analytic hierarchy process(AHP)and entropy weight method(EWM)were combined to establish a multi-index comprehensive evaluation method(AHP-EWM method).Finally,the multi-component ultrasonic extraction process of Acanthopanax senticosus was optimized by single factor combined with response surface met-hodology(RSM)based on Box-Behnken design(BBD).The results revealed that the optimal process of ultrasonic extraction of multiple components of Acanthopanax senticosus obtained by RSM optimization was as follows:Ultrasonic power 780 W,ultrasonic time 17.5 min,ethanol volume fraction 57%,solid-liquid ratio 1:40 g:mL,raw material particle size 80 mesh.Therefore,the contents of chlorogenic acid,syringin,eleutheroside E,isofraxidin,caffeic acid and sesamin in rhizome of Acanthopanax senticosus were 2235.841±12.17,517.959±6.09,861.247±5.30,66.657±1.22,45.745±0.77,99.355±0.69μg/g,respectively.The comprehensive score based on the AHP-EWM method was 95.45±0.39,which was close to the predicted theoretical value.The effective development of quantitative analysis method and efficient extraction technology of Acanthopanax senticosus have laid a foundation for its resource utilization and pharmacodynamic basic research.