肺结核病理特征及结核杆菌检测技术对比

Pathological features of pulmonary tuberculosis and the comparison of detection techniques of tuberculosis bacilli

目的探究肺结核的病理特征并比较2种常见结核分枝杆菌检测技术在诊断中的效果对比。方法收集厦门大学附属第一医院杏林分院病理科诊断的42例肺结核的临床病理资料。观察组织形态,分析肺结核的病理特征及2种常见结核分枝杆菌检测技术的效果对比。结果肺结核病理表现为类上皮样肉芽肿性炎症,部分伴干酪样坏死。结核分枝杆菌荧光定量聚合酶链反应(PCR)法的阳性率高于抗酸染色(62%和41%),且差异有统计学意义(P<0.05)。结论肺结核存在特征的组织病理学改变,结合结核分枝杆菌荧光PCR检测和抗酸染色可提高诊断效率。

Objective To explore the pathological characteristics of tuberculosis and compare the diag-nostic effects of two common mycobacterium tuberculosis detection techniques.Methods The clinicopathologic data of 42 cases of pulmonary tuberculosis diagnosed in the Department of Pathology of Xinglin Branch,the First Affiliated Hospital of Xiamen University were searched.The histological morphology of pulmonary tuberculosis was observed,the pathological characteristics of pulmonary tuberculosis were analyzed and the effectiveness of two common mycobacterium tuberculosis detection techniques were compared.Results The pathological manifesta-tions of pulmonary tuberculosis were epithelioid granulomatous inflammation,with some caseous necrosis.The pos-itive rate of fluorescence quantitative PCR for Mycobacterium tuberculosis was higher than that of acid fast staining(62%vs 41%),and the difference was statistically significant(P<0.05).Conclusion There are characteristic histopatho-logical changes in pulmonary tuberculosis.The combination of Mycobacterium tuberculosis fluorescence PCR and acid-fast staining can improve diagnostic efficiency.