雌性大鼠盲肠结扎穿孔与盲肠结扎切除脓毒症模型表型的比较

Comparison of Phenotypes of Female Rat Sepsis Models Induced by Cecal Ligation and Puncture and Cecal Ligation and Dissection

目的探讨雌性大鼠盲肠结扎切除(CLD)与盲肠结扎穿孔(CLP)脓毒症模型模拟脓毒症患者表型的差异。方法将31只成年雌性SD大鼠根据脓毒症建模方式不同,随机分为CLP组(n=21)和CLD组(n=10),采用动物代谢分析系统测量两组大鼠的体温、产热量、耗氧量、CO_(2)产生量、呼吸商、摄食量与饮水量;采用酶联免疫吸附试验(ELISA)法测定两组血清脂多糖(LPS)浓度。结果CLD组血清LPS浓度、术后16 h内总饮水量,术后第8、9、10、11 h体温,术后第9 h产热量,术后第5、9、10 h耗氧量以及术后第5、8、9、13 h CO_(2)产生量均高于CLP组(P<0.05)。结论在体温、产热量、耗氧量等代谢表型及血清LPS浓度方面,CLD脓毒症模型大鼠比CLP脓毒症模型大鼠更接近脓毒症患者表型。

Objective To explore the differences in clinical sepsis phenotypes simulated by female rat sepsis models induced by cecal ligation and puncture(CLP)and cecal ligation and dissection(CLD).Methods Thirty-one healthy adult female Sprague Dawley(SD)rats were randomly divided into CLP group(n=21)and CLD group(n=10),and were modeled by corresponding surgeries.The body temperature,heat production,oxygen consumption,CO_(2) production,respiratory quotient,food intake,and water intake of the two groups of rats were monitored by Columbus Instuments'Comprehensive Lab Monitoring System.Serum lipopolysaccharide(LPS)concentration was determined by enzyme-linked immunosorbent assay(ELISA).Results The Serum LPS concentration,total water intake in 16 h after surgery,body temperature at 8th,9th,10th,11th h after surgery,metabolic heat production at 9th h after surgery,oxygen consumption at 5th,9th and 10th h after surgery,CO_(2) discharge at 5th,8th,9th,13th h after surgery were higher in CLD group than those in CLP group(P<0.05).Conclusion In terms of metabolic phenotypes such as body temperature,heat production,and oxygen consumption and serum LPS concentration,CLD-induced sepsis model rats were more similar to clinical sepsis patients than CLP-induced sepsis model rats.