植物中松脂醇-落叶松脂素还原酶催化特征研究进展

Research Progress on Catalytic Characteristics of Pinoresinol-lariciresinol Reductase in Plants

松脂醇-落叶松脂素还原酶(PLR)是植物中木脂素生物合成的关键酶,能够催化松脂醇转化为落叶松脂素,并进一步催化落叶松脂素生成开环异落叶松脂素,且存在底物立体选择性,是一种NADPH依赖型还原酶。PLR的催化产物位于不同类型8-8′木脂素的源头,其底物选择性直接决定木脂素的骨架类型,如呋喃、二苄基丁烷、二苄基丁内酯和芳基四氢萘木脂素。因此,PLR的催化特性和表达特征在植物木脂素组成及其生物活性多样性中发挥重要作用。该文综述了PLR在植物木脂素生物合成中的作用、对映异构体选择性及其催化机制,以期为进一步研究PLR基因的生物学功能以及催化机制奠定基础,并为不同类型木脂素的精确生物合成指明方向。

Pinoresinol-lariciresinol reductases(PLRs)are key enzymes involved in the lignan biosynthesis in plants,which convert pinoresinol to lariciresinol and then to secoisolariciresinol.PLRs are NADPH-dependent reductases with substrate stereoselectivity.The catalytic products of PLR are the sources of different types of 8-8′lignans,and the substrate selectivity directly determines the skeleton types of lignans,such as furano,dibenzylbutane,dibenzylbutyrolactone and aryltetrahydronaphthalene lignans.Therefore,the catalytic and expression characteristics of PLRs play an important role in the composition and biodiversity of lignans in plants.This paper reviewed the research progress on the important role of PLRs in lignans biosynthesis,as well as its enantioselectivity and catalytic mechanism,thus to lay the foundation for further study on the biological function and catalytic mechanism of PLR genes,and point out the direction for the precise biosynthesis of different types of lignans enantiomers through synthetic biology.