RSRC1通过靶向调控PTEN/PI3K/AKT通路抑制食管鳞状细胞癌的增殖和转移

RSRC1 inhibits the proliferation and metastasis of ESCC by targeting PTEN/PI3K/AKT pathway

目的 探索富含精氨酸和丝氨酸的卷曲螺旋1(RSRC1)在食管鳞状细胞癌(ESCC)增殖和转移中的作用。方法 通过生信分析检测磷酸酶及张力蛋白同源物(PTEN)在ESCC中的表达。利用qRT-PCR和蛋白免疫印迹法检测ESCC细胞中RSRC1的表达。通过CCK-8、细胞迁移和侵袭实验阐明RSRC1对ESCC细胞增殖和转移的作用。通过蛋白免疫印迹法分析PTEN/PI3K/AKT信号通路相关因子的表达。结果 生信分析结果显示PTEN在ESCC组织中低表达。分子实验显示RSRC1在ESCC细胞中低表达。细胞实验发现敲低RSRC1可促进ESCC增殖和转移,并且可以调节PTEN/PI3K/AKT信号通路。结论 RSRC1通过靶向调控PTEN/PI3K/AKT通路抑制ESCC的增殖和转移,RSRC1可能是一种新的ESCC诊断标志物和治疗靶点。

Objective To explore the role of arginine-and serine-rich coiled coil 1(RSRC1)in the proliferation and metastasis of esophageal squamous cell carcinoma(ESCC).Methods The expression of PTEN in ESCC was detected by biogenic analysis.The expression of RSRC1 in ESCC cells was detected by qRT-PCR and Western blot(WB).The effect of RSRC1 on the proliferation and metastasis of ESCC cells was elucidated by CCK-8,cell migration and invasion tests.The expression of related factors in PTEN/PI3K/AKT signaling pathway was analyzed by WB.Results The biogenic analysis showed that PTEN was low in ESCC tissue.Molecular experiments showed low expression of RSRC1 in ESCC cells.Cell experiments showed that RSRC1 knockdown can promote ESCC proliferation and metastasis,and can regulate PTEN/PI3K/AKT signaling pathway.Conclusion RSRC1 inhibits ESCC proliferation and metastasis through targeted regulation of PTEN/PI3K/AKT pathway.RSRC1 may be a new diagnostic marker and therapeutic target for ESCC.