二穗短柄草SPL家族基因的鉴定、系统发育和表达分析

Identification,Phylogeny and Expression Analysis of the SPL Gene Family in B.distachyon.

植物特异性SPL家族基因编码SBP(squamosa promoter-binding protein-like)保守结构,参与调节植物的生长和发育。为了解二穗短柄草中SPL家族基因的分布、结构及功能,对二穗短柄草全基因组中SPL家族成员进行了系统分析。结果表明,在二穗短柄草全基因组中鉴定出17个BdSPL基因,在5条染色体上呈不均匀分布,bd03号染色体上分布最多;根据系统发育树,17个BdSPL被分为6个组,同组成员有保守的结构和基序;其中6对BdSPL基因发生基因复制事件;在BdSPL基因启动子区域发现了各种顺式元件,如ABRE、CGTAC-motif和LTR。转录组数据和qRT-PCR分析显示,BdSPL基因在二穗短柄草花中表达量较高,而在根系中表达量较低,在不同植物激素处理下表达量有差异。

The plant-specific SPL family(squamosa promoter-binding protein-like)gene encodes the SBP conserved domain that is involved in the regulation of plant growth and development.To understand the distribution,structure and function of SPL genes in Brachypodium distachyon,the SPL gene family was analyzed systematically in thewhole genome in this study.The results showed that 17 BdSPL genes were identified in the whole genome of Brachypodiumdistachyon.According to the phylogenetic tree,the 17 BdSPL genes were divided into six groups,and members of the same family shared the conserved gene structures and motifs.Gene duplication events occurred in 6 pairs of BdSPL genes.Promoter analysis showed that there were various cis-elements,such as ABRE,CGTAC-motif,and LTR.Transcriptome and qRT-PCR analysis revealed that the expression level of SPL gene was higher in flowers,but lower in roots,and different expression patterns were observed under different plant hormone treatments.