耐低温吸附态PAHs降解真菌的筛选与鉴定

Screening and identification of adsorbed PAHs degrading fungi with cold-resistance

为了从环境中筛选耐低温PAHs降解真菌,从某炼油厂冻融土壤中筛选出一株以腐殖酸(HA)吸附态PAHs为碳源和能源且生长良好的耐低温真菌(命名为JDR7),经鉴定为高山被孢霉(Mortierella alpina),并采用修复实验研究了其对冻融土壤中HA吸附态PAHs的降解性能。结果表明:JDR7对冻融土壤中Phe和Pyr均具有降解能力,42d降解率分别为80.70%和68.13%;HA的添加,可提高降解率,42 d后JDR7对冻融土壤中Phe和Pyr降解率分别提高12.3%和13.8%;若要在寒冷地区选择Mortierella alpina JDR7作为固定化真菌,可添加HA提高其修复效果。本研究为北方寒冷地区土壤PAHs污染微生物修复提供新的菌株。

In order to screen low-temperature-resistant PAHs-degrading fungi from the environment,a well-grown low-temperature-resistant fungus(named JDR7)with humic acid(HA)-adsorbed PAHs as carbon source and energy was screened from the freeze-thawed soil sample from a certain oil refinery.It was identified as Mortierella alpina,and its degradation performance of HA-adsorbed PAHs in freeze-thawed soil was studied by remediation experiments.The results showed that JDR7 had the ability to degrade Phe and Pyr in freeze-thawed soil,and the degradation rates were 80.70%and 68.13%,respectively after 42 days.The addition of HA could increase the degradation rate.After 42 days,the degradation rates of Phe and Pyr in freeze-thawed soil increased by 12.3%and 13.8%,respectively.If Mortierella alpina JDR7 is selected in cold regions as a immobilised strain,addition of HA can improve its remediation efficiency.This study provides new immobilized strain for remediation of PAH-contaminated soil in northern cold regions.