云南省怒江州独龙牛内阿米巴原虫的感染情况调查

Infection Investigation of Entamoeba spp.in Bos frontalis in Nujiang Prefecture,Yunnan Province

为了解云南省怒江州独龙牛内阿米巴原虫的感染情况,按照春、夏、秋、冬4个季节采集到的625份独龙牛粪便样本,用常规PCR方法,基于内阿米巴原虫的18S rRNA基因对粪便样本中虫体基因进行扩增。结果显示,检出内阿米巴原虫阳性样本共61份,总感染率为9.76%(61/625)。其中,鸠门当村、亚左洛村、古泉村、茨开镇、独龙江乡的感染率分别为4.12%(4/97)、7.59%(6/79)、11.18%(17/152)、13.54%(31/229)和4.41%(3/68),5个采样点之间,其感染率差异显著(P<0.05)。怒江流域与独龙江流域的内阿米巴原虫感染率分别为10.41%(58/557)和4.41%(3/68),差异不显著(P>0.05)。春、夏、秋、冬4个季节,独龙牛内阿米巴原虫的感染率分别为11.93%(26/218)、23.66%(22/93)、3.33%(4/120)和4.64%(9/194),差异极显著(P<0.01)。在阳性样本中,有51份鉴定为Entamoeba bovis,10份鉴定为Entamoeba sp.MG107/BEL。结果表明,云南省怒江州独龙牛存在内阿米巴原虫感染,应给予重视并建议采取有效措施进行防控。

In order to understand the infection of Entamoeba spp.in Bos frontalis in Nujiang prefecture,Yunnan province,total of 625 fecal samples were collected in the spring,summer,autumn and winter,and fecal sample DNA were amplified by conventional PCR methods based on the 18S rRNA gene of Entamoeba spp.The results showed that 61 samples were positive for Entamoeba spp.,the overall infection rate of Entamoeba spp.in Bos frontalis was 9.76%(61/625),and the infection rates of Entamoeba spp.were 4.12%(4/97),7.59%(6/79),11.18%(17/152),13.54%(31/229)and 4.41%(3/68)in Jiumendang village,Yazuoluo village,Guquan village,Cikai Township and Dulongjiang Township,respectively,with significant differences between the five sampling sites(P<0.05).The infection rates of Entamoeba spp.in the Nujiang River basin and N'Mai River basin were 10.41%(58/557)and 4.41%(3/68),respectively,with non-significant differences(P>0.05).The infection rates of Entamoeba spp.during spring,summer,autumn and winter were 11.93%(26/218),23.66%(22/93),3.33%(4/120)and 4.64%(9/194),respectively,with highly significant differences(P<0.01).51 positive samples were identified as Entamoeba bovis and 10 positive samples were identified as Entamoeba sp.MG107/BEL.The results of the study suggested that Entamoeba infections are prevalent in Bos frontalis in Nujiang prefecture,Yunnan province,which should be taken seriously and effective measures should be recommended for prevention and control.