德宏奶水牛FASN基因乳腺组织表达及生物信息学分析

Expression and Bioinformatics Analysis of FASN Gene in Mammary Tissue of Dehong Dairy Buffalo

【目的】研究德宏奶水牛乳腺组织FASN基因表达水平及水牛FASN蛋白的生物学特性。【方法】用乳成分分析仪测定乳脂率,并按照乳脂率将德宏奶水牛分为高(H)、中(M)、低(L)组;运用qPCR法测定乳腺组织FASN基因的表达水平,分析其与乳脂率的相关性;运用生物信息学方法分析水牛FASN蛋白的理化性质及结构特征。【结果】H组德宏奶水牛乳腺组织的FASN基因表达水平极显著高于L组和M组(P<0.01),M组极显著高于L组(P<0.01),其mRNA表达水平与乳脂率呈极显著正相关(P<0.01)。水牛FASN蛋白的分子式为C_(12188)H_(19327)N_(3363)O_(3631)S_(109),分子质量为274.56 ku,蛋白不稳定;在细胞内主要分布于细胞核;具有2个结构域,均与脂类合成代谢有关;二级结构以α-螺旋和无规则卷曲为主;进化树及同源性分析显示:水牛FASN蛋白与牦牛和家牛的亲缘关系较近,序列同源性较高;FASN主要与ACACB、ACACA和ACLY互作较为紧密。【结论】德宏奶水牛乳腺组织中FASN基因表达可能促进乳脂率提高。

[Purpose]To study the expression level of FASN gene in mammary tissue of Dehong dairy buffalo and the biological characteristics of FASN protein in buffalo.[Methods]Milk fat percentage was detected by milk composition analyzer.High,medium and low groups(groups H,M,and L)were divided by different milk fat percentage.The expression level of FASN gene in mammary tissue was detected by qPCR.The correlation of FASN gene expression level and milk fat percentage were analyzed.Physicochemical property and structural characteristics of FASN protein of buffalo were analyzed by bioinformatics method.[Results]The expression level of FASN gene in mammary tissue of Dehong dairy buffalo in group H was extremely significantly higher than that in groups L and M(P<0.01),and group M was extremely significantly higher than that in group L(P<0.01).The expression level of FASN was extremely significant positive correlated with the milk fat percentage(P<0.01).The molecular formula of buffalo FASN protein was C_(12188)H_(19327)N_(3363)O_(3631)S_(109),molecular weight was 274.56 ku.This protein was unstable,and mainly distributed in the nucleus.There were two domains in FASN,of which were all related to lipid anabolism.The secondary structure mainly consisted ofα-helix and random coil.The evolutionary tree and homology analysis showed that the FASN of buffalo was closely related to the yak and domestic cattle.The FASN mainly interacted closely with ACACB,ACACA and ACLY.[Conclusion]FASN gene expression in mammary tissue can promote the increase of milk fat percentage.