动物源菌株对7种消毒剂的抗药性分析

Analysis of resistance of animal derived strains to seven disinfectants

【目的】探明不同种类的消毒剂对动物源菌株的消毒效果。【方法】将宠物收容站、肉鸡养殖场和鹌鹑养殖场来源采集的样品进行分离培养和生化鉴定;采用微量肉汤法测定7种常用消毒剂对不同动物源菌株的最小抑菌浓度(MIC);通过PCR方法检测动物源菌株携带的消毒剂抗性相关基因。【结果】从动物源样品中分离鉴定得到1株Escherichia coli、Pasteurella、Staphylococcus aureus、Streptococcus和3株Salmonella。药物敏感性试验结果表明鹌鹑源Salmonella typhimurium F35对部分阳离子表面活性剂类、过氧化物类消毒剂抗药;宠物源Staphylococcus aureus和Streptococcus对多种消毒剂抗药性增加。抗药基因检测鹌鹑源Salmonella F35菌株携带mer A、int I1、qac EΔ1基因,宠物源Staphylococcus aureus检测出teh A基因,宠物源Streptococcus检测出teh A和int I1基因。【结论】鹌鹑源Salmonella typhimurium F35通过整合子以及外排泵等机制介导其对阳离子表面活性剂类消毒剂耐受;宠物源Staphylococcus aureus和Streptococcus通过外排泵机制介导对阳离子表面活性剂类、含氯消毒剂以及季铵盐类消毒剂耐受。

[Objective]To investigate the disinfection effects of different types of disinfectants on animal derived strains.[Methods]Separate,cultivate,and biochemical identify samples collected from pet shelters,broiler farms,and quail farms;Determine the minimum inhibitory concentration(MIC)of 7 commonly used disinfectants against different animal derived strains using the micro broth method;Detect disinfectant resistance related genes carried by animal derived strains using PCR method.[Results]One Escherichia coli,Pasteurella,Staphylococcus aureus,Streptococcus,and three Salmonella strains were isolated and identified from animal source samples.The drug sensitivity test results showed that Salmonella typhimurium F35 from quail was resistant to some cationic surfactants and peroxides as disinfectants;Pet derived Staphylococcus aureus and Streptococcus have increased resistance to various disinfectants.Drug resistance gene detection of Salmonella typhimurium F35 strain from quail carrying mer A,int I1,qac EΔ1.The teh A gene was detected by Staphylococcus aureus from pet origin,while the teh A and int I1 genes were detected by Streptococcus from pet origin.[Conclusion]Quail derived Salmonella typhimurium F35 mediates its tolerance to cationic surfactants through integrons and efflux pumps mechanisms;Pet derived Staphylococcus aureus and Streptococcus mediate tolerance to cationic surfactants,chlorine containing disinfectants,and quaternary ammonium salt disinfectants through efflux pump mechanism.