一株铜绿微囊藻溶藻真菌的分离鉴定及溶藻特性

Isolation and identification of a algolytic fungus for Microcystis aeruginosa and its algolytic characteristics

从富营养化水体和土壤中分离筛选出一株溶藻真菌菌株JHQ3,通过ITS序列分析对溶藻真菌JHQ3进行鉴定,并研究了该菌株对铜绿微囊藻的溶藻方式和溶藻特性。结果表明:菌株JHQ3的ITS序列与萨氏曲霉属(Aspergillus sydowii)同源性达100%,菌株JHQ3属于萨氏曲霉属(GenBank登录号为OP363844);溶藻真菌JHQ3对铜绿微囊藻的作用方式以直接溶藻作用为主,间接溶藻作用为辅;在30℃时菌液溶藻效果最好,溶藻率高达94.06%;在pH为7时,溶藻率最高,为83.39%;菌液浓度大于10^(-3)(即稀释10~3倍)时,其溶藻效果随菌液浓度的增加而增强;光暗比为12 h∶12 h时,溶藻效果较好,具有较好的工程应用价值;添加表面活性剂乙二胺四乙酸和Tween-80会抑制溶藻效果。溶藻菌株JHQ3能有效抑制铜绿微囊藻繁殖,在富营养化治理方面具有较好的应用前景。

An algolytic fungus strain JHQ3 was isolated and screened from eutrophic water and soil.The algolytic fungus JHQ3 was identified by ITS sequence analysis.The algolytic mode and algolytic characteristics of this strain on Microcystis aeruginosa were studied.The results showed that the ITS sequence of strain JHQ3 was 100% homology with Aspergillus sydowii,and strain JHQ3 was identified as Aspergillus sydowii(GenBank accession number OP363844).The function mode of algolytic fungus JHQ3 on Microcystis aeruginosa was mainly direct algolytic function and secondary indirect algolytic function.At 30 ℃,the algolytic effect was the strongest,with the algolytic rate up to 94.06%.When pH was 7,the algyolytic rate was 83.39%.When the concentration of bacterial solution was more than 10~(-3),the algolytic effect was enhanced with the increase of bacterial solution concentration.When the light-dark period ratio was 12 h∶12 h,the algolytic rate was better,which had good engineering application value.The addition of surfactants EDTA and Tween-80 inhibited the algolytic effect.All the results stated above indicated that the algolytic fungus strain JHQ3 can inhibit the growth of Microcystis aeruginosa efficiently and have a prospective application in the control of Microcystis aeruginosa in eutrophic water.