NIR控制的复合型纳米MOF用于耐药菌灭活

NIR-Controlled MOF Nanoconjugates for Drug-Resistant Bacteria Inactivation

伴随抗生素的大规模使用甚至滥用,细菌耐药性问题日趋严重。发展快速有效的耐药菌灭活方法对遏制耐药菌的传播至关重要。基于此,首先制备了一种富有叠氮基团的金属有机骨架(UiO MOF)。其次,通过在其中组装客体分子吲哚菁绿(ICG)和全氟己烷(PFH),外层修饰细菌靶向适配体(aptamer),构建了复合型MOF纳米材料(UiO/ICG/PFH@aptamer,缩写为UIP@Apt)。结果表明:在近红外光(NIR)照射下,UIP@Apt可用于耐药菌的光热和自供氧光动力协同灭活,体外杀菌率达96.5%,并可有效抑制生物膜的形成。该方法简单有效,长期副作用小且不产生显著耐药性,可为耐药菌的灭活提供有益参考。

With the large-scale use and abuse of antibiotics,bacterial resistance is becoming more and more serious.Developing rapid and effective inactivation methods for drug-resistant bacteria is of great significance to curb their spread.Therefore,in our current study a nano metal-organic frameworks rich in azide groups(UiO MOF)was first prepared.Then,the nano-MOF were loaded with guest molecules including indocyanine green(ICG)and perfluorohexane(PFH),and further modified with bacteria-targeted aptamers,resulting MOF nanoconjugates called UIP@Apt(simplified from UiO/ICG/PFH@aptamer).The results showed that under the irradiation of near-infrared light(NIR),the as-prepared UIP@Apt could be used for the synergistic inactivation of drug-resistant bacteria via photothermal effect as well as oxygen self-supplied photodynamic therapy.In this regard,an in vitro bactericidal rate of 96.5%could be reached,and the formation of biofilms could also be effectively inhibited.This method is simple,effective,exhibiting low long-term side effects,and does not produce obvious bacterial resistance,which may provide a new methodology for the inactivation of drug-resistant bacteria.