2020年绍兴市水产品污染与临床病例中副溶血性弧菌相关性分析

Correlation analysis of Vibrio parahaemolyticus from contaminated aquatic products and clinical cases in Shaoxing in 2022

目的了解2020年绍兴市副溶血性弧菌水产品分离株和临床分离株的血清型、耐药表型和分子分型特征及其相关性。方法选取2020年绍兴地区检出的66株以甲鱼和螺类为主的水产品分离株和67株临床分离株,使用玻片凝集法对分离株进行血清分型,采用微量肉汤稀释法测定分离株对9种抗生素的耐药性,采用脉冲场凝胶电泳(PFGE)法对分离株进行聚类分析。结果66株水产品分离株分为9个血清群和35种血清型,优势血清型为O2:K28(18.18%,12/66);67株临床分离株分为4个血清群和10种血清型,优势血清型为O3:K6(47.76%,32/67)。水产品分离株对9种测试抗生素存在不同程度的耐药,临床分离株仅对氨苄西林耐药;水产品分离株对氨苄西林耐药率(19.70%,13/66)略高于临床分离株耐药率(11.94%,8/67),且存在2株多重耐药菌株。52株水产品分离株分为51种带型,50个带型簇;63株临床分离株分为32种PFGE带型,17个带型簇;115株水产品分离株和临床分离株分为83种带型,67个带型簇。结论绍兴市以甲鱼和螺类为主的水产品中副溶血性弧菌血清群复杂多样,临床分离株耐药情况较水产品分离株轻,水产品和临床分离株之间分子分型相似度较低,无交叉聚集现象,需进一步扩大水产品监测范围,更加准确地确定污染源,降低由水产品引起的副溶血性弧菌食源性疾病的发生。

Objective To explore the serotypes,drug-resistance phenotypes,molecular characteristics,and relevance of Vibrio parahaemolyticus(Vp)from aquatic and clinical isolates procured in 2020 from Shaoxing.Methods A total of 66 aquatic isolates(mainly soft-shelled turtles and spiral shells)and 67 clinical isolates of Vp were collected in 2020 from Shaoxing.Serotype detection was performed using slide agglutination.Drug susceptibility of nine kinds of antibiotics was acquired using the broth microdilution method.Clustering analysis was performed using pulsed field gel electrophoresis(PFGE).Results The 66 aquatic isolates were divided into 9 serogroups and 35 serotypes.Moreover,O2:K28 was the dominant serotype,accounting for 18.18%(12/66).The 67 clinical isolates were divided into 4 serogroups and 10 serotypes,and O3:K6 was the dominant serotype,accounting for 47.76%(32/67).The aquatic isolates were resistant to nine kinds of antibiotics at different degrees,while the clinical isolates were only resistant to ampicillin.The resistance rate of aquatic isolates to ampicillin was 19.70%(13/66),which was marginally higher than that of clinical isolates(11.94%,8/67),with two multidrug-resistant strains.A total of 52 aquatic isolates were divided into 51 band types and 50 band clusters.Moreover,63 clinical isolates were divided into 32 band types and 17 band clusters.The 115 strains isolated from aquatic and clinical isolates were divided into 83 band types and 67 band clusters.Conclusion The Vp serogroups in aquatic isolates from soft-shelled turtles and spiral shells in Shaoxing are complex and diverse.The drug resistance of clinical isolates was lower than that of aquatic isolates.The similarity of molecular typing between aquatic isolates and clinical isolates was low,and there was no cross-aggregation.Therefore,the monitoring scope of aquatic products should be further expanded,the source of pollution more accurately determined,and the occurrence of foodborne diseases of Vp caused by aquatic products reduced.