玉米B3基因家族全基因组鉴定及表达模式

Genome wide identification and expression pattern analysis of B3 gene family in maize

B3基因家族是植物中特有的转录因子家族,在植物生长发育及胁迫响应中有重要作用。为探究B3基因家族在玉米(Zea mays)中的基本信息及生长发育和逆境胁迫中的生物学功能,本研究对玉米全基因组B3家族成员进行鉴定并分析相关信息,依据转录组数据分析B3基因家族组织特异性表达及胁迫处理下的表达响应。结果显示,玉米B3基因家族包含88个成员,分为ABI、ARF、HSI、RAV和REM 5个亚家族,均含有B3结构域,同一个亚族成员的基因结构及保守基序(数量、类型)相似。分析发现,B3成员基因的启动子区域中均富含激素及逆境响应等功能元件。组织特异性表达分析结果显示,大部分B3基因在分生组织中优势表达。对B3家族成员在冷、热、盐、紫外和干旱这5种胁迫因子的响应表达分析显示,5个亚家族成员均可不同程度参与响应冷、热、盐、紫外和干旱胁迫因子,其中HSI亚家族中的所有成员均参与了对紫外胁迫因子的响应途径。以上结果阐明了B3基因家族在玉米全基因组中的分布、结构和系统发育特征,并依据玉米转录组数据,初步推测其在生长发育和胁迫应激响应中发挥重要作用,为深入研究B3基因家族在玉米中的功能与分子机制提供了数据基础。

The B3 gene superfamily is a unique plant transcription factor family that plays an important role in plant growth,development,and stress response.In order to explore the basic information of B3 gene family in maize(Zea mays)and its biological functions in growth,development and stress,this study identified and analyzed the whole genome B3 family members and related information,and analyzed the tissue-specific expression of B3 gene family and its response to stress based on transcriptome data.The tissue specific expression of the B3 gene family and the expression pattern under stress were analyzed based on transcriptome data.Eighty-eight B3 gene family members containing B3 domains were identified in maize,and these were divided into five subfamilies:ABI,ARF,HSI,RAV,and REM.Members within the same subfamily had similar gene structures and the number and type of conservative motifs.Additionally,we discovered that the promoter regions of B3 genes were rich in functional elements that responded to hormones and stress factors.The tissue specific expression analysis results showed that most of the B3 genes were predominantly expressed in the meristem.The analysis of the expression patterns of the B3 family members under cold,heat,salt,UV,and drought stress showed that five subfamily members participated in responses to these conditions,and all members of the HSI subfamily were involved in the response to the UV stress factor.These results elucidate the distribution,structure and phylogenetic characteristics of the B3 gene family in the whole genome of maize,and preliminarily speculate that the B3 gene family plays an important role in growth and development and stress response based on the transcriptome data of maize,providing data basis for further study of the function and molecular mechanism of B3 gene family in maize.