一测多评法同时测定都梁丸中7种成分的含量

Simultaneous Determination of 7 Components in Duliang Pills by QAMS

目的建立一测多评法同时测定都梁丸中阿魏酸、洋川芎内酯Ⅰ、水合氧化前胡素、白当归素、欧前胡素、洋川芎内酯A、异欧前胡素7种成分的含量。方法采用高效液相色谱法,色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱,流速:1.0 mL/min,柱温:30℃;检测波长:300 nm。以欧前胡素为内参物,计算各成分的相对校正因子及含量,并与外标法比较。结果7个成分分别在各自范围内线性关系良好(r>0.9990),平均加样回收率在98.87%~101.34%之间(RSD<3.0%);一测多评法测定的7种成分含量的计算值与外标法实测值无明显差异。结论一测多评法简单准确可行,可用于都梁丸多指标成分的含量测定。

Objective To establish the QAMS method for simultaneous determination of 7 components in Duliang pills,including ferulic acid,ligustrolide I,oxypeucedanin hydrate,byakangelicin,imperatorin,ligustrolide A and isoimperatorin.Methods The HPLC method was performed on Diamonsil C18 column(250 mm×4.6 mm,5μm).Acetonitrile-0.1%phosphoric acid solution was used as the mobile phase by gradient elution,and the flow rate was 1.0 mL/min.The column temperature was 30℃.Detection wavelength was 300 nm.Using imperatorin as internal reference,the relative correction factors and contents of each component were calculated and QAMS method was compared with the external standard method.Results The 7 compositions had good linear relationships within their respective ranges(r>0.9990),and the average recovery rate was 98.87%-101.34%(RSD<3.0%).There was no significant difference between the calculated values of 7 components measured by QAMS and those by the external standard methods.Conclusion The QAMS method is simple,accurate and feasible,which can be used for the determination of multi-index components in Duliang pills.