水稻CNGCs家族的鉴定及非生物胁迫诱导表达模式分析

Identification of CNGCs Gene Family in Rice and Its Expression Pattern Analysis Induced by Abiotic Stresses

为了解水稻CNGCs家族成员的基因结构、进化特征、亚细胞定位、启动子顺式作用元件和生物学功能,本研究利用生物信息学的手段对水稻CNGCs家族进行鉴定,运用RT-qPCR的方法分析OsCNGCs在非生物胁迫下的诱导表达模式。结果表明,水稻共有16个CNGCs,分布在1、2、3、4、5、6、9和12号染色体上。进化树分析显示OsCNGCs可分为4个大组(Ⅰ,Ⅱ,Ⅲ和Ⅳ),而Ⅳ组可以细分为Ⅳ-A和Ⅳ-B 2个亚组。OsCNGCs启动子区域存在多种顺式调控元件,包括各种类型的光响应元件、厌氧诱导响应元件、逆境相关转录因子结合元件、防御和逆境响应元件、低温响应元件以及各种植物激素响应元件等,暗示OsCNGCs在响应多种激素和非生物胁迫中可能存在重要调控作用。多种非生物胁迫诱导表达分析表明,大多数OsCNGCs基因表现出明显的差异表达。本研究为解析CNGCs家族基因在水稻应对非生物胁迫中的功能提供了参考。

To understand the gene structure,evolutionary characteristics,subcellular location,promoter cis-acting elements and biological function bioinformatics analysis were conducted to identify rice CNGCs family,and the induced expression pattern of OsCNGCs under abiotic stress were analyzed by RT-qPCR.A total of 16 full length rice CNGC genes were identified and distributed on chromosomes 1,2,3,4,5,6,9 and 12,which are classified into four major classes(I,II,II and IV)and two sub-groups(IV-A and IV-B).The OsCNGCs promoter contain a variety of cis-acting regulatory elements including light-responsive elements,anaerobic inducible response elements,stress-related transcription factor binding elements,defense and stress responsive elements,low temperature responsive elements,and various plant hormone responsive elements,implying their potential roles in response to various hormones and stresses.The expression analysis of various abiotic stress inducible genes showed that most OsCNGC genes were differentially expressed in response to various abiotic stresses.This study provides cues for functional investigation of CNGCs genes family in rice.