摘要
PHD-finger是一类广泛存在于真核生物中,在种子发芽、育性调节、逆境胁迫等过程中发挥重要调控作用的锌指蛋白。本研究从白菜(Brassica rapa L.)全基因组中鉴定出33个PHD-finger基因,分布于9条染色体上,但不同PHD-finger基因序列结构差异较大。6个物种72个PHD-finger转录因子的系统进化分析显示有5个亚家族分枝。基于RNA-seq数据的表达分析表明,白菜PHD-finger基因在花蕾中表达量最高,但其在不育、可育花蕾中表达有差异。白菜BrPHD08、BrPHD25、BrPHD30分别与拟南芥花粉发育相关的MMD1、SHL、MS1基因直系同源,且3个基因在不育、可育花蕾中表达差异极大,推测这3个基因可能与白菜花粉发育相关。本研究为白菜PHD-finger转录因子相关基因功能研究提供生物信息学方向,为白菜花粉发育相关基因的克隆和功能研究提供理论依据。
The plant homeodomain(PHD)finger protein is a kind of zinc-finger protein widely existing in eukaryotes and plays an important role in regulating plant growth and development.In this study,33 PHD-finger family genes were identified in the genome of Brassica rapa L.,which were distributed on nine chromosomes,and dfferent PHD-finger genes have significantly different structures.Phylogenetic analysis of 72 PHD-finger family protein from six species revealed that they could be grouped into five clusters.Based on the expression analysis of RNA-seq data,the PHD-finger family genes have the highest expression in flower of B.rapa,and have different expressions in sterile and fertile flower.BrPHDO8,BrPHD25 and BrPHD30 are orthologies of MMD1,SHL and MSI which are related with pollen development of Arabidopsis,and three BrPHD genes have significantly different expressions in sterile and fertile flower,indicating that three BrPHD genes might involve in pollen development of B.rapa.These study provided a basic information for PHD-finger family genes in B.rapa,our findings also provide theoretical basis for further functional analysis of BrPHDs and candidates genes with pollen development in B.rapa.
作者
吴秀兰
唐文武
Wu Xiulan;Tang Wenwu(School of Food&Pharmaceutical Engineering,Zhaoqing,526061;School of Life Science,Zhaoqing University,Zhaoqing,526061)
出处
《分子植物育种》
CAS
北大核心
2023年第20期6649-6658,共10页
Molecular Plant Breeding
基金
肇庆学院博士科研启动基金项目(611-612281)资助。