利用隶属函数法对不同基因型秋葵耐盐碱性的分析与评价

Analysis and Evaluation of Salt and Alkaline Tolerance of Different Genotypes of Okra by Membership Function Method

为了明确不同基因型秋葵苗期耐盐碱能力的强弱,本研究选择13个秋葵品种(系)为供试材料。以沙壤土为基质,采用盆栽的试验方法进行,当秋葵长至第3片真叶完全展开时,用含有100 mmol/L(NaCl:NaHCO_(3)=1:1)的1/2 Hoagland’s营养液进行处理,每次200 mL,每3 d处理一次,共处理3次,对照为1/2浓度的Hoagland’s营养液,处理结束后,定期定量浇水,控制花盆内水分,花盆底部放托盘,渗透水分及时返还花盆中,30 d后测试株高、植株鲜重、植株干重、可溶性糖含量、脯氨酸含量、丙二醛含量,利用隶属函数法对6项指标进行了耐盐碱性综合评价。结果表明:盐碱胁迫抑制了秋葵植株的生长,降低了株高、鲜重和干重,在一定程度上增加了可溶性糖、脯氨酸和丙二醛的含量。利用隶属函数法对各个指标的综合评价得出,耐盐碱性较强的分别为:‘日引黄秋葵’、‘JK9’、‘JK13’;耐盐碱性较弱的分别为‘绿羊角’、‘JK8’、‘JK4’。

In order to determine the salt tolerance of different genotypes of okra in seedling stage,13 okra varieties(lines)were selected as test materials.With sandy loam soil as the substrate,the pot experiment method was used.When the okra grew to the third true leaf fully developed,it was treated with 1/2 Hoagland's nutrient solution containing 100 mmol/L(NaCl:NaHCO_(3)=l:1),200 mL each time,once every 3 days,three times in total,and the control was 1/2 concentration of Hoagland's nutrient solution.After the treatment,it was watered regularly and quantitatively to control the water in the flowerpot,put a tray at the bottom of the flowerpot,and return the permeated water to the flowerpot in time,30 days later,the plant height,fresh weight,dry weight,soluble sugar content,soluble protein content and malondialdehyde content were tested.The results showed that salt alkali stress inhibited the growth of okra plants,decreased plant height,fresh weight and dry weight,and increased the content of soluble sugar,proline and malondialdehyde to a certain extent.According to the comprehensive evaluation of each index by the method of membership function,the stronger salt and alkaline tolerance were:'riyinhuangqiukui',JK9','JK13';the weak salt and alkaline resistance were:Luyangjiao',JK8',JK4'.