头花蓼纳米纤维功能敷料的药效评价

Pharmacodynamic evaluation of Polygonum capitatum nanofiber functional dressing

目的评价头花蓼纳米纤维功能敷料(P-PVP-PCL)的药效。方法利用静电纺丝技术制备P-PVP-PCL,观察其微观形貌;考察P-PVP-PCL的抑菌活性、抗氧化活性和对小鼠成纤维L929细胞存活率、黏附性及迁移率的影响。通过建立大鼠背部皮肤伤口模型,考察医用纱布敷料、空白纳米纤维敷料(PVP-PCL)和P-PVP-PCL对创面愈合率的影响,观察创面组织病变和胶原纤维沉积情况,以及创面组织中血小板内皮细胞黏附分子1(CD31)阳性血管数量和转化生长因子β(TGF-β)蛋白表达。结果制得的P-PVP-PCL表面光滑,断面处可见双层结构,对金黄色葡萄球菌和大肠埃希菌的抑制率分别为(98.88±0.66)%、(94.75±1.41)%,抗氧化活性为(83.69±1.56)%,且P-PVP-PCL组的细胞活性均显著高于对照组和PVP-PCL组(P<0.05)。与医用纱布敷料相比,P-PVP-PCL更有利于L929细胞黏附;在48 h时,该组的细胞划痕已基本愈合。与医用纱布敷料组比较,PVP-PCL组和P-PVP-PCL组的大鼠创面愈合率均显著升高(P<0.05)。干预第14天时,P-PVP-PCL组创面已基本愈合,创面组织中胶原纤维排列相对整齐、密度相对均匀;CD31阳性血管数量和TGF-β蛋白表达与干预第7天比较有下降趋势,其中CD31阳性血管数量显著低于医用纱布敷料组和PVP-PCL组(P<0.05),TGF-β蛋白表达显著高于医用纱布敷料组和PVP-PCL组(P<0.05)。结论P-PVP-PCL在体外具有良好的抗菌和抗氧化活性,能够促进L929细胞的增殖、黏附和迁移;在体内能够促进大鼠伤口愈合。

OBJECTIVE To evaluate the efficacy of the functional dressing of Polygonum capitatum nanofibers(P-PVP-PCL).METHODS P-PVP-PCL were prepared by electrospinning technology,and the microstructure of P-PVP-PCL was observed.The antibacterial activity and antioxidant activity of P-PVP-PCL and its effects on the survival rate,adhesion and migration rate of mouse fibroblast L929 cells were investigated.The effects of medical gauze dressing,blank nanofiber dressing(PVP-PCL)and P�PVP-PCL on the healing rate of the wound were investigated by establishing the back skin wound model of rats.The pathological changes of the wound tissue and collagen fiber deposition were observed,as well as the number of platelet endothelial cell adhesion molecule-1(CD31)positive blood vessels and the expression of transforming growth factor-β(TGF-β)protein in wound tissue.RESULTS P-PVP-PCL had a smooth surface and a double-layer structure at the cross-section.The inhibition rates of P-PVP-PCL against Staphylococcus aureus and Escherichia coli were(98.88±0.66)%and(94.75±1.41)%,respectively.The antioxidant activity of P-PVP-PCL was(83.69±1.56)%,and the cell activity of the P-PVP-PCL group was significantly higher than those of the control group and PVP-PCL group(P<0.05).Compared with medical gauze dressings,P-PVP-PCL was more conducive to L929 cell adhesion;at 48 hours,the cell scratches in this group had basically healed.Compared with the medical gauze dressing group,the wound healing rates of the PVP-PCL group and the P-PVP-PCL group were significantly increased(P<0.05).On the 14th day of intervention,the wounds in the P-PVP-PCL group had basically healed,there was no dermal necrosis in the wound tissue,and the collagen fibers were arranged relatively neatly and the density was relatively uniform.The number of CD31 positive blood vessels and the expression of TGF-βprotein showed a downward trend compared with the 7th day of intervention,and the number of CD31 positive blood vessels was significantly lower than those of the medical gauze dressing group and PVP-PCL group(P<0.05),but the protein expression of TGF-βwas significantly higher than those of the medical gauze dressing group and the PVP-PCL group(P<0.05).CONCLUSIONS P-PVP-PCL has good antibacterial and antioxidant activity in vitro,and can promote the proliferation,adhesion and migration of L929 cells.It can promote wound healing of rats in vivo.