基于高分辨质谱和网络药理学的可可茶多酚降血糖活性研究

Studying on the hypoglycemic activity of green tea polyphenols from Camellia ptilophylla Chang using high-resolution mass spectrometry and network pharmacology

目的:探究可可茶茶多酚的组成特性及功能活性。方法:以可可茶多酚粗提物为研究材料,采用高分辨质谱分析并鉴定其组分;基于网络药理学预测其潜在功能。结果:经酶抑制试验证实可可茶具有显著抑制α-葡萄糖苷酶和α-淀粉酶活性的体外降血糖活性;通过GO功能分析和KEGG通路富集预测其作用机制,涉及GAPDH、AKT等6个关键靶点和PI3K-AKT等多条通路,通过构建“组分—靶点—通路”网络,结合各组分与淀粉水解酶结合的分子对接筛选出杨梅素、花青素等10种核心降血糖活性组分。结论:可可茶多酚粗提物通过多组分、多靶点、多通路可以共同发挥降血糖功效。

Objective:This study aimed to explore the composition and the functional bioactivity of tea polyphenols from cocoa tea.Methods:Chemical components of crude green tea polyphenol extracts from cocoa tea were analyzed and identified using high resolution mass spectrometry.Additionally,their potential functional effects were predicted by network pharmacological analysis.Results:The in vitro hyperglycemic potential of crude green tea polyphenol from cocoa tea was preliminarily confirmed based on their strong inhibition onα-glucosidase andα-amylase.Six key targets including GAPDH and AKT and multiple pathways such as PI3K-AKT were predicted to be involved in the underying mechanisms behind the hyperglycemic effects of crude green tea polyphenol from cocoa tea through the GO enrichment and KEGG enrichment analysis.Ten polyphenol components including myricetin,anthocyanin,etc.were suggested to play a key role in the hyperglycemic activities of crude green tea polyphenol from cocoa tea via the construction of a“component-target-pathway”network combined with the molecular docking between these components and starch hydrolase.Conclusion:Crude green tea polyphenols from cocoa tea exerted a high hypoglycemic effect by regulating multi-targets through multi-pathways via multi-components.