四种桑黄的培养特性及主要活性成分的比较分析

Comparison Analysis of Cultural Character and Main Active Components of Four Sanghuangporus

以金桑黄JS1、龙桑黄LS2、杨桑黄YS3和桑黄S6 4种桑黄菌种为试材,采用紫外比色法比较不同桑黄子实体中总三萜、总黄酮和总多糖的含量,通过对桑黄母种、原种和子实体栽培进行优选,分析不同阶段各桑黄菌丝体生长情况及子实体中活性成分的差异,以期为桑黄功能活性物质的研究及子实体品质控制提供参考依据。结果表明:不同阶段菌丝萌发速度呈显著性差异,4种桑黄母种均在以蔗糖为碳源的PDA加富培养基上生长最快,在玉米粉和麸皮培养基上生长相对缓慢,而在桑枝滤液培养基上生长最慢,YS3菌丝生长表型综合最佳。桑黄原种菌丝在第3天的生长速度均最快,JS1、YS3和S6在麸皮培养基中菌丝生长均最快,分别为4.78、4.60 mm·d^(-1)和3.08 mm·d^(-1);LS2在4种培养基中生长速度差异不显著,其中在玉米粉培养基中最快为4.17 mm·d^(-1)。对比分析不同桑黄子实体活性成分含量,LS2子实体多糖含量最高,YS3子实体总黄酮和三萜含量最高,S6子实体黄酮、三萜及多糖含量均最低。综合考虑菌丝生长速度、农艺性状、子实体活性成分含量,桑黄YS3和LS2均可作为优良的生产菌株。

Four species of Phellinus,Sanghuangporus vaninii JS1,Sanghuangporus vaninii LS2,Inonotus vaninii YS3 and Phellinus igniarius S6 were optimized through the optimization of Sanghuangporus parent species,original species and fruiting bodies cultivation.The Sanghuangporus lindenus mycelial growth and fruiting bodies active components were analysed for these four species.The content of total triterpenes,flavonoids and polysaccharides extracts in fruiting bodies were compared by ultraviolet colorimetric colorimetry,in order to provide reference for the study of functiomal active substances and the quality control of fruiting bodies.The results showed that there were significant difference in mycelial germination rates at different stages were observed.The growing of four Sanghuangporus species on PDA-enriched medium with sucrose as carbon source were the fastest.The growth was relatively slower on the corn meal and bran medium,and on the mulberry branch filtrate medium was the slowest.The growth phenotype of Inonotus vaninii YS3 mycelium was the best in terms of growth on the mulberry branch filtrate medium.The growth of Sanghuangporus philoxeroides were the fastest after the third day of culture,JS1,YS3 and S6 mycelial grew the fastly in bran medium,and the growth rates were 4.78 mm·d^(-1),4.60 mm·d^(-1) and 3.08 mm·d^(-1),respectively.There was no significant difference in the growth rate of LS2 in the four media,among which the fastest was 4.17 mm·d^(-1) in the corn meal medium.The content of polysaccharides in the fruit body of LS2 was the highest,the total flavonoids and triterpenes in YS3 fruiting bodies were the highest,and the contents of flavonoids,triterpenes and polysaccharides in S6 fruiting bodies were all lowest.Inonotus vaninii YS3 and Sanghuangporus vaninii LS2 could be used as excellent production strains considering the growth rate of mycelial,agronomic characters and active component content of fruiting bodies.