基于ISSR分子标记的胡枝子属亲缘关系研究

Genetic Relationship Analysis of Lespedeza Based on ISSR Molecular Markers

以29份胡枝子属植物叶片为试材,采用简单序列重复区间(inter-simple sequence repeat,ISSR)分子标记技术进行试验,并构建指纹图谱,研究了29份胡枝子属植物材料间的亲缘关系及遗传多样性,以期为华北地区胡枝子属植物的引种和育种工作提供参考依据。结果表明:共筛选出10条多态性ISSR引物,利用多态性ISSR引物进行PCR扩增,共扩增出93条谱带,其中多态性条带92条,多态性比例(PPB)为98.92%,并选取引物UBC-812构建指纹图谱。平均有效基因数为1.4775,平均Nei′s遗传多样性指数(H)为0.2933。通过UPGMA聚类分析,在遗传相似性系数为0.61时,可将29份材料分为3个类群,与传统分类并不完全一致。综上可知,胡枝子属植物遗传多样性较高,二色胡枝子实生筛选品种并未完全聚在一起,说明通过杂交育种等方法培育的二色胡枝子的遗传多样性得到显著提高。

The genetic diversity and genetic relationship of 29plant materials of Lespedeza were studied by inter-simple sequence repeat(ISSR) molecular marker technology,and fingerprints were constructed,in order to provide reference for the introduction and breeding of Lespedezain North China.The results showed that a total of 10 polymorphic ISSR primers were screened,PCR amplification was performed by polymorphic ISSR primers,and a total of 93bands were amplified,included 92polymorphic bands,and the polymorphism ratio(PPB)was 98.92%,and primer UBC-812was selected to construct a fingerprint.The average effective gene number was 1.477 5and the average Nei′s genetic diversity index(H)was 0.293 3.Through UPGMA cluster analysis,when the genetic similarity coefficient was 0.61,the 29 materials could be divided into 3 groups,which was not completely consistent with the traditional classification.In conclusion,the genetic diversity of Lespedeza was high,and the selected varieties of Lespedeza bicolor were not completely clustered,indicated that the genetic diversity of Lespedeza bicolor cultivated by cross-breeding and other methods had been significantly improved.