QSOX1在骨肉瘤中的表达及其作用机制

The expression of QSOX1 in osteosarcoma and its functional study

目的研究QSOX1在骨肉瘤组织和细胞中的表达及其在细胞增殖、迁移、侵袭中的作用。方法Western blot及免疫组化实验验证QSOX1在骨肉瘤组织及细胞中的表达情况并选出表达更高的细胞系MG63。慢病毒敲低并筛选出稳定株shQSOX1组和shCtrl组,Western blot检测转染后MG63中QSOX1蛋白表达水平;CCK-8实验检测细胞增殖水平;划痕实验验证细胞迁移水平;Transwell实验检测细胞侵袭能力。Western blot检测shCtrl组和shQSOX1组中NF-κB信号通路mRNA表达水平。结果Western blot及免疫组化结果表明QSOX1在人成骨细胞系hFOB1.19以及癌旁组织中低表达,而在骨肉瘤组织以及骨肉瘤细胞中高表达(P<0.001)。CCK-8实验显示在shQSOX1组中,其增殖能力较shCtrl组受到抑制(P<0.05);划痕实验中,shQSOX1组迁移能力减弱(P<0.001);Transwell实验中,shQSOX1组侵袭能力受到影响(P<0.001);NF-κB在shCtrl组高表达,在shQSOX1组低表达(P<0.001)。结论QSOX1在骨肉瘤中高表达,敲除QSOX1基因可以抑制骨肉瘤细胞的增殖、迁移、侵袭能力,且敲除QSOX1使得NF-κB信号通路失活。

Objective To study the expression of QSOX1 in osteosarcoma tissues and cells and its role in proliferation,migration and invasion.Methods Western blot and immunohistochemistry were used to verify the expression of QSOX1 in osteosarcoma tissues and cells,and the cell line MG63 with the highest expression was selected.Slow virus knocked down and selected stable strains shQSOX1 group and shCtrl group.Western blot was used to detect the expression level of QSOX1 protein in MG63 after transfection.CCK-8 assay was used to detect the level of cell proliferation.Scratch test verified the level of cell migration.Transwell test was used to detect the invasion ability of cells;Western blot was used to detect the mRNA expression level of NF-κB signal pathway in shCtrl group and shQSOX1 group.Results Western blot and immunohistochemistry showed that QSOX1 was low expressed in human osteoblast line hFOB1.19 and adjacent tissues,but high expressed in osteosarcoma tissue and osteosarcoma cells(P<0.001).CCK-8 experiment showed that the proliferation ability of shQSOX1 group was inhibited compared with shCtrl group(P<0.05);In the scratch test,the migration ability of shQSOX1 group decreased(P<0.001);Transwell proved that the invasive ability of shQSOX1 group was affected(P<0.001);NF-κB was highly expressed in shCtrl group and low expressed in shQSOX1 group(P<0.001).Conclusion QSOX1 is highly expressed in osteosarcoma.Knockout of QSOX1 gene can inhibit the proliferation,migration and invasion of osteosarcoma.Knockout of QSOX1 makes NF-κB signal pathway is deactivated.