大鼠肾皮质淋巴管内皮微通道

The lymphatic endothelial microchannel in the renal cortex of the rat

目的探查大鼠肾皮质淋巴管内皮微通道及其超微结构。方法获取6只大鼠的12个肾脏,对其中8个肾脏进行组织切片、苏木精-伊红(HE)和免疫组织化学(IHC)染色检查。另4个经处理后作扫描电镜检测。结果大鼠肾脏的HE染色切片很难发现具特征性的淋巴管管壁,但通过LYVE-1的IHC染色可见其呈散在分布,外形不规则,管壁菲薄,并以不同的形状及大小存在,有些细胞核突入管腔中。扫描电镜观察,大鼠肾皮质中淋巴管外表可见散在分布的内皮微通道入口,其包含半圆形的“穹隆顶部”和平坦的“底部”,代谢产物散布在其周围或聚集在入口处。结论初步介绍了大鼠肾皮质淋巴管内皮微通道的形态结构,其结果可为进一步的基础研究、组织工程和3D组织打印以及临床应用提供实验动物的形态学理论基础。

Objective To explore the endothelial microchannel and ultrastructure of the lymphatic vessel in the renal cortex of the rat.Methods Twelve kidneys were obtained from six rats.Eight of them were examined by histological section,Haematoxylin Eosin(HE)and Immunohistochemical(IHC)staining.The other four were examined by scanning electron microscopy after tissues were pre-prepared.Results Lymphatic vessels were hardly recognized in the HE staining section of the rat kidney,but could be detected by IHC staining of LYVE-1.They had irregular and thin walls with varying shapes and sizes.Some nuclei of the endothelial were protruded into the lumen.The scanning electron microscope showed that scattered entrances of the endothelial microchannel were seen on the lymphatic vessel in the renal cortex of the rat,which contained a semicircular“roof”and a flat“floor”.Metabolites were scattered around or gathered at the entrance.Conclusion This study introduces the morphological structure of endothelial microchannels of the lymphatic vessel in the renal cortex of the rat.The result can provide a morphological theoretical basis of experimental animals for further basic scientific research,tissue engineering,3D tissue printing,and clinical applications.