高油酸花生油酶促酯交换制备1,3-二棕榈酸-2-油酸甘油三酯

Preparation of 1,3-dipalmitoyl-2-oleoylglycerol with high-oleicp eanut oil by enzymatic transesterification

旨在为可可脂生产提供新的基料油,同时为高油酸花生油的高值化利用提供研究基础,以高油酸花生油和棕榈酸乙酯为原料,脂肪酶NS40086为催化剂,在无溶剂体系下酶法合成1,3-二棕榈酸-2-油酸甘油三酯(POP)。采用单因素实验研究反应条件对POP含量、酰基位移、sn-2位油酸相对含量的影响,并采用响应面法进行优化。结果表明:高油酸花生油酶促酯交换制备POP的最佳反应条件为反应时间3 h、底物(棕榈酸乙酯与高油酸花生油)物质的量比11∶1、酶添加量(以底物总质量计)3%、反应温度50℃,在此条件下POP含量为86.48%,酰基位移为3.25%,sn-2位油酸相对含量为72.88%。

In order to provide new base oil for the production of cocoa butter and research basis for the high-value utilization of high-oleic peanut oil,with high-oleic peanut oil and ethyl palmitate as raw materials,NS40086 lipase as catalyst,1,3-dipalmitoyl-2-oleoylglycerol(POP)was synthesized by enzymatic transesterification in a solvent-free system.The effects of reaction conditions on the POP content,acyl migration and the relative content of sn-2 oleic acid were studied by single factor experiment,and response surface methodology was used to determine the optimal reaction conditions.The results showed that the optimal reaction conditions were obtained as follows:reaction time 3 h,molar ratio of ethyl palmitate to high-oleic peanut oil 11∶1,dosage of enzyme 3%(based on total substrates mass),reaction temperature 50℃.Under these conditions,the POP content,acyl migration and the relative content of sn-2 oleic acid were 86.48%,3.25%and 72.88%,respectively.