复合菌分解低品位磷矿研究

Solubilization of Low-Grade Phosphate Rock by Complex Bacteria

为实现低品位磷矿资源利用,探究复合菌的解磷作用。将4株解磷菌,假单胞菌LA、棘孢木霉LZ1、巨大芽孢杆菌EC3、沙雷氏菌LX1进行了组合,筛选出解磷效果最佳的复合菌。研究了不同碳源、氮源及复合菌组合方式等因素对复合菌解磷效果的影响,利用单因素和正交实验优化了复合菌分解低品位磷矿的条件。研究表明复合菌LA+EC3解磷效果最好,可溶磷质量浓度达到397.86 mg/L;蔗糖和硫酸铵分别为复合菌LA+EC3的最适碳源和氮源,先单菌培养再组合的方式解磷效果更好;复合菌分解低品位磷矿的最优条件为:复合菌接种量10%、蔗糖质量浓度10 g/L、硫酸铵质量浓度0.5 g/L、初始pH 5。结果表明,复合菌的解磷效果并不都优于单菌株,不同复合菌组合方式、初始接种量、初始pH、碳源和氮源对复合菌解磷效果有显著影响。

To realize the utilization of low-grade phosphorus ore resources,the phosphorus-solubilizing effect of complex bacteria was explored.Four phosphate-solubilizing strains,Pseudomonas LA,Trichoderma aculeatus LZ1,Bacillus megaterium EC3 and Serratia LX1,were combined to select the complex bacteria with the best effects on phosphorus solubilization.The effects of different carbon sources,nitrogen sources and the combination of complex bacteria on phosphorus solubilization were studied,and the conditions for solubilization of low-grade phosphate ore by complex bacteria were optimized by using single-factor and orthogonal experiments.The study showed that the complex bacteria LA+EC3 have the best effect on phosphorus solubilization,and the mass concentration of soluble phosphorus reaches 397.86 mg/L.Sucrose and ammonium sulfate are the most suitable carbon and nitrogen sources for the complex bacteria LA+EC3.The optimal conditions for the solubilization of low-grade phosphorus ore by the complex bacteria are as follows:10%inoculum of complex bacteria,sucrose mass concentration of 10 g/L,ammonium sulfate mass concentration of 0.5 g/L,and initial pH of 5.The results showed that the phosphorus solubilization effects of complex bacteria are not always better than that of single strains,and different combinations of complex bacteria,initial inoculum,initial pH,carbon and nitrogen sources have significant effects on the phosphorus solubilization effect of complex bacteria.