去壁灵芝孢子粉水溶成分抑制头颈鳞癌细胞增殖及转移

Aqueous-soluble components of sporoderm-removed Ganoderma lucidum spore powder inhibit the proliferation and metastasis of head and neck squamous cell carcinoma

目的探讨去壁灵芝孢子粉水溶成分(A-GSP)在头颈鳞癌细胞中的生物学作用。方法利用MTS法检测A-GSP在头颈鳞癌细胞系SCC15和SCC25中的细胞活力。采用平板克隆、划痕愈合实验和穿室实验探究A-GSP对头颈鳞癌细胞增殖、转移能力的影响。通过流式分析试剂盒检测A-GSP对头颈鳞癌细胞凋亡能力和细胞周期分布的影响。免疫蛋白印迹法检测A-GSP处理后上皮间质转化(EMT)分子和细胞周期蛋白的表达变化。结果A-GSP作用后,头颈鳞癌细胞的增殖能力减弱(F=62.72,P<0.0001),转移能力下降(F=95.7,P<0.0001),细胞凋亡能力增加(F=87.65,P<0.0001),G2/M期停滞(F=15.31,P<0.01)。此外,A-GSP处理导致上皮细胞标志物E-cadherin上调,间质细胞标志物Vimentin、N-cadherin、Snail、Slug下调,细胞周期激酶CDK1下调,P21和P27上调,但是,并未引起凋亡通路蛋白Caspase和Bax水平改变。结论A-GSP通过诱导细胞周期G2/M期停滞和抑制EMT从而抑制头颈鳞癌细胞的增殖转移。

Objective To investigate the biological role of aqueous-soluble components of sporoderm-removed Ganoderma lucidum spore powder(A-GSP)in head and neck squamous cell carcinoma(HNSCC).Methods The effects of A-GSP on the proliferation and metastasis of head and neck squamous cell carcinoma cells SCC15 and SCC25 were investigated using MTS,plate cloning,scratch healing experiments,and chamber penetration experiments.The effect of A-GSP on the apoptosis ability and cell cycle distribution of head and neck squamous cell carcinoma cells was detected using a flow cytometry assay kit.Western blot was used to detect the expression changes of epithelial mesenchymal transformation(EMT)molecules and cell cycle proteins after A-GSP treatment.Results After the action of A-GSP,the proliferation ability of head and neck squamous cell carcinoma cells decreased(F=62.72,P<0.0001),the metastasis ability decreased(F=95.7,P<0.001),the apoptosis ability increased(F=87.65,P<0.001),and the G2/M phase stagnated(F=15.31,P<0.01).In addition,A-GSP treatment resulted in upregulation of epithelial cell markers E-cadherin,downregulation of mesenchymal cell markers Vimentin,N-cadherin,Snail,Slug,downregulation of cell cycle kinase CDK1,and upregulation of P21 and P27.However,it did not cause changes in the levels of apoptosis pathway proteins Caspase and Bax.Conclusion A-GSP inhibits the proliferation and metastasis of head and neck squamous cell carcinoma cells by inducing G2/M phase arrest and inhibiting EMT.