吸烟对急性缺血性脑卒中患者淋巴细胞亚群的影响

Effect of Smoking on the Lymphocyte Subsets in Patients with Acute Ischemic Stroke

目的:探讨吸烟对急性缺血性脑卒中患者外周免疫细胞的影响。方法:纳入165例发病在72 h内的急性缺血性脑卒中患者,收集基线临床和实验室数据。采集外周血标本,采用流式细胞术分析淋巴细胞亚群的数量和百分比,并将吸烟者与非吸烟者进行比较。对不同参数进行单因素和多因素Logistic回归分析。采用Spearman分析来评估吸烟强度与淋巴细胞亚群之间的相关性。结果:在急性缺血性卒中患者中,与非吸烟者相比,吸烟者的B细胞数量(P<0.001)和百分比更高(P=0.009),总T细胞(P=0.013)和抑制性T细胞数量更高(P=0.032)。单因素Logistic回归分析显示,吸烟与B细胞数量(OR=3.384,95%CI 1.621~7.067,P=0.001)和百分比(OR=2.243,95%CI 1.095~4.596,P=0.027)、T细胞数量(OR=2.104,95%CI1.055~4.196,P=0.035)显著相关。相关性分析显示,吸烟强度与B细胞数量、B细胞百分比、T细胞数量呈正相关(P值分别为0.002、0.009、0.012)。结论:吸烟是导致急性缺血性脑卒中患者外周血B细胞数量和百分比增加、T细胞数量增加的独立危险因素。

Objective:To investigate the influence of smoking on peripheral immune cells in patients with acute ischemic stroke.Methods:A total of 165 patients with stroke onset within 72 hours were enrolled in the present study.Baseline clinical and laboratory data were collected.Peripheral blood samples were obtained,and the count and percentages of lymphocyte subsets were analyzed by flow cytometry,allowing for comparisons between individuals who smoked or not.Univariate and multivariate logistic regression analyses were performed for various parameters.Spearman analysis was employed to assess the correlation between smoking intensity and lymphocyte subsets.Results:In patients with acute ischemic stroke,smokers exhibited notably higher count(P<0.001)and percentage(P=0.009)of B cells,as well as an elevated count of T cells(P=0.013)and suppressor T cells(P=0.032)when compared to non-smokers.Univariate logistic regression analysis showed a significant association between smoking and count(OR=3.384,95%CI 1.621~7.067,P=0.001)and percentage(OR=2.243,95%CI 1.095~4.596,P=0.027)of B cells,as well as T cell count(OR=2.104,95%CI 1.055~4.196,P=0.035).Furthermore,correlation analysis revealed a positive association between smoking intensity and the count of B cells(P=0.002),the percentage of B cells(P=0.009),and the count of T cells(P=0.012).Con⁃clusion:Smoking was an independent risk factor of higher count and percentage of B cells,and a higher count of T cells in patients with acute ischemic stroke.