基于全基因组的温州光唇鱼源大肠杆菌E105的致病性与耐药性解析

Pathogenicity and Drug Resistance Analysis of Escherichia coli Strain E105 from Micropterus salmoides Based on Whole Genome

为了加深对鱼源大肠杆菌(Escherichia coli)的理解,以全基因组测序为基础,解析了温州光唇鱼(Acrossocheilus wenchowensis)源大肠杆菌E105致病性和耐药性的表型与基因型间的关系,以期丰富大肠杆菌的病原学知识。结果显示,E105全基因组包括染色体基因组(5 022 520 bp)和6个质粒基因组,共预测到4 823个编码基因,4 510个基因被COG聚类到22类,1 140个基因富集到KEGG的22条代谢通路。E105与人(Homo sapiens)源(GenBank ID CP068822.1)和鸭(Anatinae)源(GenBank ID JAGFYD010000001)大肠杆菌基因组共线性程度更高。E105血清型为O108:H9,E105为EAEC/EHEC/EPEC混合致病型。E105中有170个毒力基因。E105对小鼠(Mus musculus)和大口黑鲈(Micropterus salmoides)的半数致死浓度(median lethal dose,LD50)分别为6.67×10^(5) CFU/g和2.61×10^(7) CFU/g。E105中有84个耐药基因。E105对糖肽类的万古霉素和替考拉宁、大环内酯类的克拉霉素和红霉素、林可酰胺类的克林霉素和林可霉素、硝基咪唑类的甲硝唑、四环素类的四环素和多西环素、头孢菌素类的头孢氨苄和头孢噻肟、氨基糖苷类的庆大霉素、利福霉素类的利福平、复方磺胺类的复方新诺明、甲氧苄啶类的甲氧苄啶、青霉素类的青霉素G和氨苄西林共11类17种抗生素耐药。总之,本研究中的鱼源多重耐药性致病性大肠杆菌E105存在一定的公共卫生风险。

To deepen the understanding of Escherichia coli from fish,based on the whole genome sequencing,the relationship between the phenotype and genotype of pathogenicity and drug resistance of E.coli E105 from Acrossocheilus wenchowensis was analyzed,so as to enrich the pathogenic knowledge of E.coli.The results showed that the E105 whole genome consisted of a chromo-somal genome(5022520 bp)and 6 plasmid genomes,with a total of 4823 coding genes predicted.4510 genes were clustered into 22 categories by COG,and 1140 genes were enriched into 22 metabolic pathways of KECG.E105 had higher collinearity with Homo sapiens(GenBank ID CP068822.1)and Anatinae(GenBank ID JACFYD010000001)Escherichia coli genomes.The E105 serotype is O108:H9,and E105 belongs to the EAEC/EHEC/EPEC mixed pathogenic type.There are 170 virulence genes in E105.The median lethal dose(LDso)of E.coli E105 for Mus musculus and Micropterus salmoides was 6.67×10^(5)CFU/g and 2.61×10^(7) CFU/g,respectively.There are 84 drug resistance genes in E105.E105 has resistance to 11 classes and 17 antibiotics,such as vancomycin and teicoplanin of glycopeptides,clarithromycin and erythromycin of macrolide,clindamycin and lincomycin of lincomamides,metronidazole of nitroimidazoles,tetracyclines and doxycycline of tetracyclines,cefalexin and cefotaxime of cephalosporin,gentamicin of aminoglycoside,rifampicin of rifampicin,trimethoprim/sulfamethoxazole of compound sulfa,trimethoprim of benzaminopyrimidines,penicillin G and ampicllin of penicillins.In summary,the multi-drug resistant pathogenic E.coli E105 from fish sources in this study poses certain public health risks.