昆虫Kr-h1基因的生物信息学分析

Bioinformatic Analysis of Insect Kr-h1 Gene

Kr-h1基因是研究昆虫变态发育与害虫生物防治的重要靶标分子。本研究解析了昆虫Kr-h1基因结构、保守结构域、二级结构与三级结构,开展了多序列比对、表达模式分析与系统发育树重构。结果表明,Kr-h1基因外显子数量较为保守,多为2个,而内含子长度变异较大。Kr-h1蛋白一般含8个锌指结构,二维与三维结构显示锌指结构保障了蛋白的稳定性。基于全长序列的系统发育分析显示,昆虫Kr-h1为直系同源,亲缘关系与物种进化相吻合,基于锌指结构的系统发育则显示Kr-h1不同模块进化中的差异。菜粉蝶转录组数据显示,该基因表达模式与变态密切相关。本研究丰富了昆虫Kr-h1基因的知识,以期为菜粉蝶、西花蓟马等昆虫变态发育研究与生物防治提供新策略。

Kr-h1(Krüppel homolog 1)is a key molecular target in the regulation of insect metamorphosis and development,and in biological pest control.In this study,insect Kr-h1 gene structure,conserved domain,secondary and tertiary structure,multiple sequences alignment and expression pattern are analyzed,as well as phylogenetic trees are reconstrued.Results indicating that,exon number of Kr-h1 gene containing two exons in most insects,is conserved,while intron in Kr-h1 gene is various in length.Most insects Kr-h1 proteins contain zinc finger structures,which provide the stability of Kr-h1 protein from secondary and tertiary structure evidence.Phylogenetic trees indicate that insect Kr-h1 genes are orthologous among insect species,and genetic relationship of protein fits insect species evolution.However,different motifs of zinc finger structures showed diversities based on phylogenetic tree.Transcription of Pieris rapae data showed close relationship of expression pattern and metamorphosis.Our results enrich knowledge of insect kr-h1gene,providing a new strategy for metamorphosis,development and biological control of P.rapae and Frankliniella occidentalisa.