基于cfDNA的NGS技术探究术后脓毒症患者血液微生物群特征

Characteristic of blood microbiota of postoperative sepsis based on cfDNA NGS

目的分析外科手术术后脓毒症患者血液微生物群特征。方法从SRA-NCBI公共数据库下载239例外科手术术后脓毒症患者(SP)和34例术后未感染患者[对照患者(CP)]血浆样本中细胞游离DNA(cfDNA)的原始测序数据,采用生物信息学方法进行再次分析。结果与CP比较,SP血液样本微生物多样性显著下降。SP血液样本中检测到12类显著富集的物种,其中相对丰度较高的代表性菌种有6类:麦芽寡养单胞菌、红串红球菌、食酸菌sp.KKS102、皮氏罗尔斯顿菌、屎肠球菌和人乙型疱疹病毒5。结论外科手术术后SP血液内微生物组成改变明显,有6类代表性物种可能在脓毒症的发展过程中起重要作用。

Objective To analyze the characteristic of blood microbiota in patients with sepsis after operation.Methods The sequencing data of microbial cell-free DNA(cfDNA)from plasma of 239 postoperative sepsis patients(SP)(SP group)and 34 postoperative patients without SP[control patients(CP)(CP group)]were downloaded from SRA-NCBI databases and reanalyzed by bioinformatics.Results The blood microbiota in SP group showed a decreased microbial diversity compared with CP group,which mainly enriched in 12 taxa.The most representative taxa which were increased in SP group,with higher relative abundance,included Stenotrophomonas maltophilia,Rhodococcus erythropolis,Acidovorax sp.KKS102,Ralstonia pickettii,Enterococcus faecium and human beta herpes virus 5.Conclusions The composition of blood microbiota is altered in SP group compared with CP group,and the 6 representative taxa may play roles in the development of SP.