肺癌组织与血液中DHX32的表达水平及临床意义

The Expression Level and Clinical Significance of DHX32 in Lung Cancer Tissue and Blood

目的 分析肺癌组织与血液中DHX32的表达水平及临床意义。方法 研究开展时间为2020年8月至2021年10月,研究对象为厦门医学院附属第二医院99例肺癌患者(纳入肺癌组)、28例肺良性结节患者(纳入肺良性结节组)、100例健康体检人群(纳入健康体检人群组),2020年8月取得肺良性结节、肺癌病理标本,检验DHX32,开展预试验,同年9月以健康体检者为参照,扩大试验研究范围,进一步分析DHX32在肺癌诊治中的应用意义。采用免疫组织化学法测量肺癌组织和肺良性结节中的DHX32(包括DHX32a和DHX32b);借助ELISA方法测量血清中的DHX32;经由化学发光法或电化学法检测3组血清中的CEA、CYFRA21-1、NSE、SCC和ProGRP,分析DHX32等指标在肺癌检验中的结果,探讨临床意义。结果 肺癌组组织中的DHX32a和DHX32b均明显高于肺良性结节组(P<0.05);健康体检人群组、肺良性结节组和肺癌组血清中的DHX32a和DHX32b均呈现出明显升高趋势(P<0.05);健康体检人群组、肺良性结节组和肺癌组的CEA、CYFRA21-1、NSE、SCC和ProGRP均呈现出明显升高趋势(P<0.05)。结论 肺癌组织、血液中DHX32高表达,通过测量DHX32,能有效预测肺部病变情况,同时可以鉴别肺部疾病性质,区分良恶性病灶,为疾病诊疗提供重要参考信息。

Objective To analyze the expression level and clinical significance of DHX32 in lung cancer tissue and blood.Methods The study will be conducted from August 2020 to October 2021.The study subjects are 99 patients with lung cancer(included in the lung cancer group),28 patients with benign pulmonary nodules(included in the benign pulmonary nodules group),and 100 healthy people undergoing physical examination(included in the healthy physical examination group).In August 2020,pathological samples of benign lung nodules and lung cancer were obtained,and DHX32 was tested for preliminary experiments.In September of the same year,healthy individuals were used as reference to expand the scope of the experimental study,further analyze the application significance of DHX32 in the diagnosis and treatment of lung cancer.Using immunohistochemistry to measure DHX32(including DHX32a and DHX32b)in lung cancer tissue and benign pulmonary nodules;Measure DHX32 in serum using ELISA method;Detect CEA,CYFRA21-1,NSE,SCC,and ProGRP in three groups of serum through chemiluminescence or electrochemical methods,analyze the results of DHX32 and other indicators in lung cancer testing,and explore clinical significance.Results The levels of DHX32a and DHX32b in the lung cancer group were significantly higher than those in the benign nodules group(P<0.05).The serum levels of DHX32a and DHX32b in the health examination population group,benign pulmonary nodules group,and lung cancer group showed a significant upward trend(P<0.05).The CEA,CYFRA21-1,NSE,SCC,and ProGRP levels in the health examination population group,benign pulmonary nodules group,and lung cancer group showed a significant upward trend(P<0.05).Conclusion DHX32 is highly expressed in lung cancer tissue and blood.By measuring DHX32,it can effectively predict lung lesions,differentiate the nature of lung diseases,differentiate benign and malignant lesions,and provide important reference information for disease diagnosis and treatment.