前列腺癌组织中miRNA-17-3p表达及其临床价值

Expression and Value of miRNA-17-3p in Prostate Cancer Tissues

【目的】探讨前列腺癌(PCa)组织中微小RNA-17-3p(miRNA-17-3p)表达及其临床价值。【方法】选择2017年12月至2019年7月榆林市中医医院收治的92例PCa患者(观察组),另外选择同期于本院行手术治疗的92例前列腺增生患者为对照组。所有患者入院后均接受手术治疗,留取术中组织标本,检测组织中标本miRNA-17-3p的表达量,比较两组患者miRNA-17-3p表达水平。采用受试者工作特征(ROC)曲线评估miRNA-17-3p诊断PCa的临床价值;采用Pearson相关性分析miRNA-17-3p与格里森评分及PSA的相关性。【结果】观察组miRNA-17-3p表达水平高于对照组,差异有统计学意义(P<0.01)。ROC曲线分析结果显示,miRNA-17-3p诊断PCa的曲线下面积(AUC)(95%CI)为0.84(0.77~0.90),诊断界值为23.19,敏感度及特异度分别为58%、77%。高表达组中PCa患者TNM分期Ⅲ~Ⅳ期、PCa低分化所占比例及PSA、格里森评分高于低表达患者,差异有统计学意义(P<0.05)。Pearson相关性分析结果显示,miRNA-17-3p与PSA(r=0.659,P=0.036)及格里森评分(r=0.332,P=0.004)均呈显著正相关。【结论】miRNA-17-3p在前列腺癌组织中高表达,是潜在的诊断及评估PCa病情的指标。

【Objective】To analyze the expression and value of microRNA-17-3p(miRNA-17-3p)in prostate cancer(PCa)tissues.【Methods】A total of 92 PCa patients in urology were collected randomly in this study between December 2017 and July 2019 as the PCa group,meanwhile 92 patients with benign prostatic hyperplasia were selected as the control group.All of them were accepted surgery after admission and the tissues were preserved during operation,then the level of miRNA-17-3p were detected from tissues and compared with the two groups.The receiver operating characteristic(ROC)curve was used to evaluate the clinical value of miRNA-17-3p in diagnosing PCa;Pearson correlation was used to analyze the correlation between miRNA-17-3p,Gleason score and PSA.【Results】The level of miRNA-17-3p in the PCa group was significantly higher than that of the control group.ROC curve analysis results showed that the area under the curve(AUC,95%CI)of the value of miRNA-17-3p in diagnosis of PCa was 0.84(0.77-0.90),diagnostic cut-off value was 23.19,and sensitivity and specificity were 58%and 77%,respectively.The ratios of TNM stage(stageⅢ+Ⅳ),PSA,Gleason score and low differentiation of PCa in high-expression of miRNA-17-3p group were higher than those in low miRNA-17-3p expression group.Pearson correlation analysis results showed that there was a significant positive correlation of miRNA-17-3p expression with PSA(r=0.659,P=0.036)and with Gleason score(r=0.332,P=0.004).【Conclusion】The miRNA-17-3p was up-regulation in PCa,and it can be severed as a potential indicator in diagnosis and evaluation of PCa.