中西医结合研究的思维及方法学亟待创新——运用细胞行为组学进行中医药研究的探讨

Innovative Methodology on the Integration of Traditional Chinese and Western Medicine Approaches——A Brief Discussion Regarding Utilizing Cell Actomics on Traditional Chinese Medicine Research

组学技术在近年来中西医结合的发展中发挥了重要作用。由于生理或病理状态是由生命体最基本的单位:细胞的行为表现聚合而形成的,所以凭借各种组学所产生的分子信息必须在细胞层面加以整合,并延展到人体整体层面才能体现中医学理论的精髓。细胞行为是细胞内蛋白网络中所有蛋白活性受到外界调控后在细胞层面的总体表现。精细量化细胞的各种行为可以解析不同蛋白通路在细胞行为上的占比,并将各种分子组学信息和细胞行为紧密连结。笔者首次倡导基于细胞行为组学来研究中医药生物学基础的策略,旨在探寻适合中医学理论的研究方法,以全面体现中医学理论内涵及其实践在现代科学中的意义。

The omics techniques has played a significant role in the development of the integration of Chinese and Western medicine.Physiological or pathological states are formed by the aggregation of the behavior of the most basic unit of life:the cell.Molecular information that generated by the various omics and integrated at the cellular level must extend to the whole human body.Therefore,it can embody the true spirit of Chinese medicine(CM)theory.Cellular behavior is the aggregate performance of all proteins in the intracellular protein network at the cellular level after external regulation.Detailed quantification of cellular behavior can explain the proportion of different protein pathways,and link various molecular omics information closely to cellular behavior.This paper firstly advocates the strategy of studying the biological basis of CM based on cellular behavioral omics,in order to find research methods suitable for TCM theory,fully embodies the connotation of CM theory and the significance of practice in modern science.