牛至挥发油化学成分鉴定及其主要成分对临床病原菌的抑制作用研究

Identification of chemical constituents of essential oil and its inhibitory effect on clinical pathogens of main ingredients from Origanum vulgare L.

探索牛至挥发油的化学成分及其主要成分对临床病原菌的抑制作用,筛选有效抑菌活性成分。通过GC-MS联用技术,分析牛至挥发油的化学成分及其含量;利用肉汤稀释法检测牛至挥发油对临床病原菌金黄色葡萄球菌、大肠杆菌、生物被膜介导的产ESBLs耐药大肠杆菌的抑制作用;利用96微孔板法研究牛至挥发油中主要成分1,8-桉树脑对耐药大肠杆菌生物被膜形成的影响。GC-MS联用技术鉴定出牛至挥发油中共有45种化学成分,占总挥发油的99.77%,主要成分是1,8-桉树脑(20.8%)、石竹烯(10.2%)、丁香油酚甲醚(9.8%)、香茅醇乙酸酯(8.8%)。牛至挥发油对临床病原菌金黄色葡萄球菌、大肠杆菌具有一定的抑制作用,其主要成分1,8-桉树脑可显著影响耐药大肠杆菌生物被膜的形成。牛至挥发油鉴定出45种化学成分,并具有较好的抑菌活性,其中1,8-桉树脑可作为潜在的生物被膜介导的耐药菌的耐药抑制活性成分。

To explore the chemical components of essential oil from Origanum vulgare L.and its main components'inhibitory effect on clinical pathogenic bacteria,and screen effective antibacterial active components.The chemical composition and content of vola-tile oil from Origanum vulgare L.were analyzed by GC-MS.The inhibitory effect of volatile oil on clinical pathogenic bacteria which were Staphylococcus aureus,Escherichia coli and biofilm-mediated ESBLs-resistant Escherichia coli that detected by broth dilution method.The effect of 1,8-cineole,the main component of volatile oil,on the biofilm formation of drug-resistant Escherichia coli was studied by 96 micro-well plate method.45 chemical components were identified by GC-MS,accounting for 99.77%of the total vola-tile oil.The main components were 1,8-cineole(20.8%),caryophyllene(10.2%),eugenol methyl ether(9.8%),citronellol acetate(8.8%),respectively.Essential oil from Origanum vulgare L.has certain inhibitory effect on Staphylococcus aureus and Escherichia coli.Its main component,1,8-cineole,can significantly affect the formation of biofilm of drug-resistant Escherichia coli.Essential oil from Origanum vulgare L,has identified 45 chemical components and good antibacterial activity.Among them,1,8-cineole can be used as a potential active component of drug resistance inhibition of drug resistant bacteria mediated by biofilm.