不同时点急性肾缺血再灌注损伤大鼠模型的构建与评价

Establishment and evaluation of acute renal ischemia-reperfusion injury model of rats at different time points

目的:通过构建大鼠急性肾缺血再灌注损伤(IRI)模型,评价不同时点急性肾IRI大鼠的肾损伤程度。方法:将30只SPF级SD大鼠随机分成6组,每组5只,适应性饲养1周后,以2%戊巴比妥钠(30 mg/kg)腹腔注射麻醉,以背侧入径夹闭双侧肾蒂的方式构建急性肾IRI模型,不同的分组按照不同的缺血时间(0、5、15、30、45和60 min)进行处理,再灌注24 h后,腹主动脉采血2 mL用于检测肾功能(血尿素氮、血清肌酐和胱抑素C),取出肾脏,观察肾脏组织病理形态学、肾小管上皮细胞超微结构及细胞凋亡水平变化。结果:随着肾缺血时间延长,急性肾IRI模型大鼠肾功能进行性减退(P<0.05),肾组织病理损伤愈加严重(P<0.05),肾小管上皮细胞超微结构凋亡指数和细胞凋亡率显著升高(P<0.05),而大鼠肾缺血60 min再灌注的肾损伤程度最严重。结论:大鼠肾脏控制缺血时间45 min再灌注时间24 h时构建的急性肾IRI模型较为合适。

AIM:A renal ischemia-reperfusion injury(IRI)model of rats was established to evaluate the de‐gree of renal injury in rats with IRI at different time points.METHODS:A total of 30 SPF SD rats were randomly divided into 6 groups with 5 rats in each group.After 1 week of adaptive feeding,the acute renal IRI model was established by in‐traperitoneal injection anesthesia with 3%pentobarbital sodium(30 mg/kg)and a dorsal approach to clamping bilateral re‐nal pedicle.Different groups were treated according to different ischemia time(0,5,15,30,45 and 60 min).After 24 h of reperfusion,2 mL of blood was collected from the abdominal aorta for the test of renal function(blood urea nitrogen,se‐rum creatinine and cystatin C).The kidneys were removed and observed for renal histopathological morphology,ultra‐structure of renal tubular epithelial cells,and changes in the level of apoptosis.RESULTS:With the extension of renal ischemia time,the renal function of the acute renal IRI model of rats was progressively decreased(P<0.05),the renal his‐topathological injury of renal tissue was more serious(P<0.05),and the apoptosis index and apoptosis rate of the ultra‐structure of renal tubule epithelial cells were significantly increased(P<0.05).Moreover,the degree of renal injury was most severe in rats with reperfusion after 60 min of renal ischemia.CONCLUSION:The acute renal IRI model which es‐tablished at a controlled ischemia time of 45 min and reperfusion time of 24 h is more suitable.