血清卷曲螺旋蛋白49对胃癌诊断的价值

The Clinical Value of Serum CCDC49 in the Diagnosis of Gastric Cancer

目的 研究血清卷曲螺旋蛋白49(coiled-coil domain containing protein 49,CCDC49)对胃癌诊断的价值。方法 选取胃癌组患者和慢性胃炎组患者共288例,其中胃癌患者147例,慢性胃炎患者141例。采用双抗体夹心酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA)测定血清CCDC49水平,统计分析血清蛋白CCDC49的诊断灵敏度、特异性,评估血清CCDC49在胃癌诊断中的临床价值。结果 胃癌组的CCDC49水平为0.251(0.124~1.103)ng/mL,慢性胃炎组的CCDC49水平为0.158(0.080~0.386)ng/mL。萎缩性胃炎组、浅表性胃炎组的CCDC49水平分别为0.164(0.094~0.553)ng/mL和0.133(0.034~0.237)ng/mL。胃癌组血清CCDC49水平高于慢性胃炎组,差异有统计学意义(P<0.05);胃癌组CCDC49水平与浅表性胃炎组差异有统计学意义(P<0.05);胃癌组CCDC49水平与萎缩性胃炎组差异无统计学意义(P>0.05);萎缩性胃炎组CCDC49水平与浅表性胃炎组差异无统计学意义(P>0.05)。血清CCDC49的ROC曲线下面积为0.667,95%CI为0.555~0.779。当血清CCDC49水平为0.554ng/mL时,诊断胃癌的灵敏度和特异性分别为42.55%和82.93%。结论 血清CCDC49具有胃癌诊断的潜能,并优于血清CA19-9。

Objective To investigate the value of serum coiled-coil domain containing protein 49(CCDC49)in the diagnosis of gastric cancer.Methods A total of 288 patients with gastric cancer and chronic gastritis were collected for the study.Among selected patients,there were 147 cases of gastric cancer and 141 cases of chronic gastritis.Double-antibody sandwich enzyme-linked immunosorbent assay(ELISA)was used for level detection.The expression level of serum CCDC49 was determined in all patients,and the results were statistically analyzed.The receiver operating characteristic curve(ROC curve)was drawn.The sensitivity and specificity of serum coiled-coil protein 49(CCDC49)were analyzed to further evaluate the clinical value of serum CCDC49 in the diagnosis of gastric cancer.Results The expression level of serum CCDC49(0.251ng/mL)in the gastric cancer group was higher than that(0.158ng/mL)in the chronic gastritis group(P<0.05).The expression level of CCDC49 in the atrophic gastritis group was significantly different from that(0.133ng/mL)in superficial gastritis group(P<0.05).There was no significant difference in the expression level of CCDC49 between the gastric cancer group and atrophic gastritis group(P>0.05);there was no significant difference in CCDC49 expression level between the atrophic gastritis group and superficial gastritis group(P>0.05).AUC of serum CCDC49 was 0.66,and the 95%CI ranged from 0.555 to 0.779.When the optimal critical value of serum CCDC49 was 0.554ng/mL,the sensitivity and specificity of CCDC49 in the diagnosis of gastric cancer were 42.55%and 82.93%,respectively.Conclusion Serum coiled-coil protein 49(CCDC49)shows potentials as a diagnosis marker of gastric cancer and is superior to CA19-9.